Chumakov M, Voroshilova M, Shindarov L, Lavrova I, Gracheva L, Koroleva G, Vasilenko S, Brodvarova I, Nikolova M, Gyurova S, Gacheva M, Mitov G, Ninov N, Tsylka E, Robinson I, Frolova M, Bashkirtsev V, Martiyanova L, Rodin V
Arch Virol. 1979;60(3-4):329-40. doi: 10.1007/BF01317504.
Virological and serological studies of an epidemic disease in Bulgaria, 1975, were carried out. Epidemiologically, clinically and pathomorphologically, the disease simulated almost all known forms of poliomyelitis, acute stem encephalitis, encephalomyocarditis and aseptic meningitis. The studies completely rules out the participation of polioviruses and provided comprehensive evidence for the etiological role of a peculiar enterovirus subsequently identified as enterovirus (EV) type 71 known in the literature since 1974. Altogether, in 1975 and 1976 from 65 cases of poliomyelitis-like disease (PLD) 92 strains of EV71 were isolated, including 37 strains from the brain and medulla, 1 from the cerebrospinal fluid, 10 from mesenterial lymph nodes and tonsils and 44 from faeces. In addition, in 282 convalescent cases of the disease, diagnostic seroconversion or high titers of antibody to this virus were demonstrated. The most successful virus isolation was achieved by inoculation of green monkey kidney cell cultures and newborn white mice. Bulgarian strains of enterovirus 71 regularly caused paralysis in monkeys and morphological poliomyelitis-like lesions in their CNS, and paralysis and myositis with Zenker necrosis in newborn white mice, cotton rats, Syrian hamsters, and 3-week-old cotton rats. The diseased rodents had much more virus in their mucles than in brains.
1975年，保加利亚对一种流行病进行了病毒学和血清学研究。从流行病学、临床和病理形态学来看，该疾病几乎模拟了所有已知形式的脊髓灰质炎、急性脑干脑炎、脑心肌炎和无菌性脑膜炎。这些研究完全排除了脊髓灰质炎病毒的参与，并为一种特殊肠道病毒的病因学作用提供了全面证据，该病毒后来被确定为1974年以来文献中已知的肠道病毒（EV）71型。1975年和1976年，共从65例脊髓灰质炎样疾病（PLD）中分离出92株EV71，其中37株来自脑和延髓，1株来自脑脊液，10株来自肠系膜淋巴结和扁桃体，44株来自粪便。此外，在282例该疾病的康复病例中，证实有诊断性血清转化或针对该病毒的高滴度抗体。通过接种绿猴肾细胞培养物和新生小白鼠，病毒分离最为成功。保加利亚的肠道病毒71株经常导致猴子麻痹，并在其中枢神经系统中出现形态学上类似脊髓灰质炎的病变，还导致新生小白鼠、棉鼠、叙利亚仓鼠和3周龄棉鼠出现麻痹和伴有Zenker坏死的肌炎。患病啮齿动物肌肉中的病毒比脑中的多得多。
