Department of Veterinary Science, The University of Melbourne, Melbourne, Australia.
Parasite Immunol. 2012 May;34(5):265-75. doi: 10.1111/j.1365-3024.2011.01304.x.
The advent and integration of high-throughput '-omics' technologies (e.g. genomics, transcriptomics, proteomics, metabolomics, glycomics and lipidomics) are revolutionizing the way biology is done, allowing the systems biology of organisms to be explored. These technologies are now providing unique opportunities for global, molecular investigations of parasites. For example, studies of a transcriptome (all transcripts in an organism, tissue or cell) have become instrumental in providing insights into aspects of gene expression, regulation and function in a parasite, which is a major step to understanding its biology. The purpose of this article was to review recent applications of next-generation sequencing technologies and bioinformatic tools to large-scale investigations of the transcriptomes of parasitic nematodes of socio-economic significance (particularly key species of the order Strongylida) and to indicate the prospects and implications of these explorations for developing novel methods of parasite intervention.
高通量 '-omics' 技术（例如基因组学、转录组学、蛋白质组学、代谢组学、糖组学和脂质组学）的出现和整合正在彻底改变生物学的研究方式，使生物体的系统生物学得以探索。这些技术现在为寄生虫的全球分子研究提供了独特的机会。例如，对转录组（生物体、组织或细胞中的所有转录物）的研究在提供有关寄生虫中基因表达、调控和功能方面的见解方面变得至关重要，这是理解其生物学的重要一步。本文的目的是综述下一代测序技术和生物信息学工具在具有社会经济意义的寄生线虫转录组的大规模研究中的最新应用，并指出这些探索对开发寄生虫干预新方法的前景和影响。
