Faculty of Veterinary Science, The University of Melbourne, Parkville, Victoria 3010, Australia.
Biotechnol Adv. 2012 May-Jun;30(3):469-88. doi: 10.1016/j.biotechadv.2011.08.016. Epub 2011 Aug 26.
The advent and integration of high-throughput 'omic technologies (e.g., genomics, transcriptomics, proteomics and metabolomics) are becoming instrumental to assist fundamental explorations of the systems biology of organisms. In particular, these technologies now provide unique opportunities for global, molecular investigations of parasites. For example, studies of the transcriptomes (all transcripts in an organism, tissue or cell) of different species and/or developmental stages of parasitic nematodes provide insights into aspects of gene expression, regulation and function, which is a major step to understanding their biology. The purpose of this article was to review salient aspects of the systematics and biology of selected species of parasitic nematodes (particularly key species of the order Strongylida) of socio-economic importance, to describe conventional and advanced sequencing technologies and bioinformatic tools for large-scale investigations of the transcriptomes of these parasites and to highlight the prospects and implications of these explorations for developing novel methods of parasite intervention.
高通量 'omic 技术（例如基因组学、转录组学、蛋白质组学和代谢组学）的出现和整合正在成为辅助生物体系统生物学基础研究的有力工具。特别是，这些技术现在为寄生虫的全球分子研究提供了独特的机会。例如，对不同物种和/或寄生线虫发育阶段的转录组（生物体、组织或细胞中的所有转录物）的研究提供了对基因表达、调控和功能的认识，这是理解其生物学的重要一步。本文的目的是综述具有社会经济重要性的几种寄生线虫（特别是强线虫目关键种）的系统学和生物学的显著方面，描述用于这些寄生虫转录组大规模研究的常规和先进测序技术和生物信息学工具，并强调这些探索为开发寄生虫干预的新方法带来的前景和影响。
