Kalitina T A
Vopr Virusol. 1978 Sep-Oct(5):621-5.
A method for concentration of enteroviruses in meat extracts was developed using polyvinylpyrrolidone (molecular mass 10,000), polyethylene glycol (molecular mass 15,000), both in 10% concentration, silicagel with a granule size of 200 mesh in a concentration of 5%, aluminum hydroxide in an amount of centrifuged pellet of 5 ml of original suspension per 10 ml meat extract. When the optimal conditions of meat extract preparation (meat mass: Hanks' solution ration 1: 10) were observed, followed by virus concentration in polymers of silicagel 2--4 PFU of enteroviruses in 1 g of meat could be determined in all the experiments. Virus doses of 0.2--0.5 PFU per 1 g of meat could be detected in some experiments.
开发了一种在肉提取物中浓缩肠道病毒的方法，该方法使用10%浓度的聚乙烯吡咯烷酮（分子量10,000）、10%浓度的聚乙二醇（分子量15,000）、浓度为5%的200目硅胶颗粒、每10毫升肉提取物中加入相当于5毫升原始悬浮液离心沉淀量的氢氧化铝。当观察到肉提取物制备的最佳条件（肉质量：汉克斯溶液比例为1:10）时，随后在硅胶聚合物中进行病毒浓缩，在所有实验中均能测定出每1克肉中含有2 - 4个肠道病毒空斑形成单位（PFU）。在一些实验中能够检测到每1克肉中0.2 - 0.5个PFU的病毒剂量。
