Kalitina T A
Vopr Pitan. 1975 Nov-Dec(6):52-5.
A method of isolating the virus ECHO II in specimens of stewed beef and cutlets is assessed. The procedure involves obtaining extracts of the meat with the saline (in ratios of 1:2 and 1:4) in combination with processing with freon 113. This method failed to detect in the meat samples analyzed 20-30 CPD50 of the virus in 1 g. Experiments demonstrated that eluation of the poliovirus from the meat into an aqueous saline depended on the proportion of meat and the eluating solution. It was shown that the optimal extraction of the poliovirus from meat into the solution was seen to occur with the proportions of meat and the eluating solution of 1:8-1:9.
对在炖牛肉和肉饼标本中分离埃可病毒II型的方法进行了评估。该程序包括用生理盐水（比例为1:2和1:4）提取肉类，并结合使用氟利昂113进行处理。该方法未能在分析的肉类样本中检测到每克20 - 30个病毒半数细胞病变剂量（CPD50）。实验表明，脊髓灰质炎病毒从肉类洗脱到盐水溶液中取决于肉类与洗脱液的比例。结果显示，当肉类与洗脱液的比例为1:8 - 1:9时，脊髓灰质炎病毒从肉类到溶液中的最佳提取效果最为明显。
