Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, Chemistry College, Central China Normal University, Luoyu Str. 152, Wuhan 430079, Hubei Province, PR China.
Biosens Bioelectron. 2011 Jul 15;26(11):4320-5. doi: 10.1016/j.bios.2011.04.025. Epub 2011 Apr 20.
This article reports the fabrication of a nanocomposite biosensor for the sensitive and specific detection of methyl parathion. The nanocomposite sensing film was prepared via the formation of gold nanoparticles on silica particles, mixing with multiwall carbon nanotubes and subsequent covalent immobilization of methyl parathion hydrolase. The composite of the individual materials was finely tuned to offer the sensing film with high specific surface area and high conductivity. A significant synergistic effect of nanocomposites on the biosensor performance was observed in biosensing methyl parathion. The square wave voltammetric responses displayed well defined peaks, linearly proportional to the concentrations of methyl parathion in the range from 0.001 μg mL⁻¹ to 5.0 μg mL⁻¹ with a detection limit of 0.3 ng mL⁻¹. The application of this biosensor in the analysis of spiked garlic samples was also evaluated. The proposed protocol can be used as a platform for the simple and fast construction of biosensors with good performance for the determination of enzyme-specific electroactive species.
本文报道了一种用于灵敏和特异性检测甲基对硫磷的纳米复合生物传感器的制备。纳米复合传感膜是通过在硅颗粒上形成金纳米粒子，与多壁碳纳米管混合，随后共价固定甲基对硫磷水解酶来制备的。对各材料的复合材料进行了精细调整，为传感膜提供了高比表面积和高导电性。在生物传感甲基对硫磷中观察到纳米复合材料对生物传感器性能具有显著的协同效应。方波伏安响应显示出清晰的峰，与 0.001 μg mL⁻¹ 至 5.0 μg mL⁻¹ 范围内的甲基对硫磷浓度呈线性比例关系，检测限为 0.3 ng mL⁻¹。还评估了该生物传感器在加标大蒜样品分析中的应用。所提出的方案可用作简单快速构建生物传感器的平台，用于测定酶特异性电活性物质，具有良好的性能。
