School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, China.
Mikrochim Acta. 2019 Jun 13;186(7):433. doi: 10.1007/s00604-019-3493-7.
A glassy carbon electrode (GCE) was consecutively modified with amino groups and phosphate groups, and then loaded with Zr(IV) ions. Fourier transform infrared spectrophotometry, field-emission scanning electron microscopy, energy dispersive X-ray spectroscopy and cyclic voltammetry were used to characterize the morphologies and electrochemical properties. The sensor was used to detect p-nitrophenyl-substituted organophosphorus pesticides, with methyl-parathion (MP) as the model analyte. Under optimized conditions, the oxidation current of square wave voltammetry (typically measured at around -0.28 V vs. saturated calomel electrode) increases linearly in the 1.0 to 100 ng mL MP concentration range, and the detection limit is 0.25 ng mL (at a signal to noise ratio of 3). Average recoveries from (spiked) real water samples are 99.9-102.2%, with relative standard deviations of 0.3-2.6% (n = 3) at three levels. The reliability and accuracy of the method was validated by HPLC. Graphical abstract Zr(IV) modified GCE is prepared via three steps. The electrode shows high specificity and selectivity towards methyl-parathion. And the linear range is 1.0 - 100.0 ng mL- with the detection limit as low as 0.25 ng mL- with SWV.
一种玻碳电极（GCE）先后经过氨基和磷酸基修饰，然后负载锆（IV）离子。傅里叶变换红外光谱、场发射扫描电子显微镜、能量色散 X 射线光谱和循环伏安法用于表征形貌和电化学性质。该传感器用于检测对硝基苯基取代的有机磷农药，以甲基对硫磷（MP）为模型分析物。在优化条件下，方波伏安法（通常在饱和甘汞电极附近约-0.28 V 处测量）的氧化电流在 1.0 至 100 ng mL MP 浓度范围内呈线性增加，检测限为 0.25 ng mL（信噪比为 3）。从（加标）实际水样中的平均回收率为 99.9-102.2%，三个水平的相对标准偏差为 0.3-2.6%（n=3）。该方法的可靠性和准确性通过 HPLC 进行了验证。
