VITEK2系统和Sensititre YeastOne®对从血培养中分离出的酵母针对四种抗真菌药物进行抗真菌药敏试验的实验室间评估。

Interlaboratory evaluation of VITEK2 system and Sensititre YeastOne® for antifungal susceptibility testing of yeasts isolated from blood cultures against four antifungal agents.

作者信息

Farina Claudio, Manso Esther, Andreoni Stefano, Conte Marco, Fazii Paolo, Lombardi Gianluigi, Sanna Silvana, Russello Giuseppe

机构信息

Comitato Studio Micologia (CoSM), Associazione Microbiologi Clinici Italiani, Milano, Italia.

出版信息

New Microbiol. 2011 Apr;34(2):195-201. Epub 2011 Apr 30.

PMID:21617832

Abstract

An interlaboratory evaluation (seven centers) of VITEK2 System and Sensititre YeastOne® was conducted to test the antifungal susceptibilities of yeasts. The MICs of amphotericin B, fluconazole, flucytosine, and voriconazole were determined for 70 isolates of Candida spp. Our results demonstrated a higher interlaboratory agreement of VITEK 2 System than Sensititre YeastOne©. A good concordance between the two methods was observed for amphotericin B, fluconazole, voriconazole and 5-fluorocytosine (from 81.4% to 88.6%). The study suggests the potential value of the VITEK2 System as a convenient alternative method for testing the susceptibility of yeasts. It also indicates the need for further optimization of MIC endpoint criteria to improve interlaboratory agreement.

摘要

开展了一项关于VITEK2系统和Sensititre YeastOne®的实验室间评估（七个中心），以检测酵母的抗真菌药敏性。对70株念珠菌属分离株测定了两性霉素B、氟康唑、氟胞嘧啶和伏立康唑的最低抑菌浓度（MIC）。我们的结果表明，VITEK 2系统的实验室间一致性高于Sensititre YeastOne©。两性霉素B、氟康唑、伏立康唑和5-氟胞嘧啶在两种方法之间观察到良好的一致性（从81.4%至88.6%）。该研究表明VITEK2系统作为检测酵母药敏性的便捷替代方法具有潜在价值。它还表明需要进一步优化MIC终点标准以提高实验室间的一致性。