Pavlovic J, Zürcher T, Haller O, Staeheli P
Institut für Immunologie und Virologie, Universität Zürich, Switzerland.
J Virol. 1990 Jul;64(7):3370-5. doi: 10.1128/JVI.64.7.3370-3375.1990.
MxA and MxB are interferon-induced proteins of human cells and are related to the murine protein Mx1, which confers selective resistance to influenza virus. In contrast to the nuclear murine protein Mx1, MxA and MxB are located in the cytoplasm, and their role in the interferon-induced antiviral state was unknown. In this report we show that transfected cell lines expressing MxA acquired a high degree of resistance to influenza A virus. Surprisingly, MxA also conferred resistance to vesicular stomatitis virus. Expression of MxA in transfected 3T3 cells had no effect on the multiplication of two picornaviruses, a togavirus, or herpes simplex virus type 1. Treatment of MxA-expressing cells with antibodies to mouse alpha-beta interferon did not abolish the resistance phenotype. The conclusion that resistance to influenza virus and vesicular stomatitis virus was due to the specific action of MxA is further supported by the observation that transfected 3T3 cell lines expressing the related MxB failed to acquire virus resistance.
Mx A和Mx B是人类细胞中由干扰素诱导产生的蛋白质，与赋予对流感病毒选择性抗性的小鼠蛋白质Mx1相关。与核内的小鼠蛋白质Mx1不同，Mx A和Mx B位于细胞质中，它们在干扰素诱导的抗病毒状态中的作用尚不清楚。在本报告中，我们表明，表达Mx A的转染细胞系对甲型流感病毒获得了高度抗性。令人惊讶的是，Mx A还赋予了对水疱性口炎病毒的抗性。在转染的3T3细胞中表达Mx A对两种微小核糖核酸病毒、一种披膜病毒或1型单纯疱疹病毒的增殖没有影响。用抗小鼠α-β干扰素抗体处理表达Mx A的细胞并没有消除抗性表型。转染的3T3细胞系表达相关的Mx B却未能获得病毒抗性，这一观察结果进一步支持了对流感病毒和水疱性口炎病毒的抗性是由于Mx A的特异性作用这一结论。
