Spinal Cord Society Research Center, Fort Collins, CO 80526, USA.
Brain Res. 2011 Jul 5;1399:1-14. doi: 10.1016/j.brainres.2011.05.005. Epub 2011 May 31.
We have successfully removed an existing glial scar in chronically contused rat spinal cord using a rose Bengal-based phototoxic method. The purpose of this study is to examine if scar ablation benefits the anatomical recovery by cell/tissue transplantation, and thus provides a more permissive physical and biochemical environment for axonal growth, which may lead to functional recovery. Immediately after scar ablation, we transplanted lamina propria (LP) of the olfactory mucosa alone or in combination with cultured olfactory ensheathing cells (OEC) into the lesion cavity 6 weeks after contusion injury (NYU impactor device, 25 mm height setting) at spinal cord segment T10 of adult female Long-Evans rats. Sixteen weeks after scar ablation and transplantation, we found that the initial repaired tissue significantly expanded, companied by remarkable reduction or disappearance of the lesion cavity and integration of repaired tissue with the spared tissue, thus resulting in histological repair of damaged cord tissue at the injury epicenter. Glial scar reformation was effectively prevented after ablation due to the tissue repair. In addition, at the injury epicenter P0 (myelin glycoprotein P-zero)-positive myelination formed by Schwann cells, which are known to myelinate regenerating and demyelinated axons, were significantly increased in number compared with the control animals. However, when evaluated with BBB open-field scale a significant improvement of locomotor function was not observed in this study; the possible reasons were discussed.
我们已经成功地使用孟加拉玫瑰红基光毒性方法去除了慢性挫伤大鼠脊髓中的现有神经胶质瘢痕。本研究的目的是检验瘢痕消融是否通过细胞/组织移植有益于解剖学恢复，从而为轴突生长提供更允许的物理和生化环境，这可能导致功能恢复。在挫伤后 6 周（NYU 撞击器装置，25 毫米高度设置），在 T10 脊髓段，瘢痕消融后立即单独或与培养的嗅鞘细胞（OEC）一起将嗅黏膜固有层（LP）移植到损伤腔中。瘢痕消融和移植后 16 周，我们发现初始修复组织显著扩大，伴随着损伤腔的显著减少或消失以及修复组织与保留组织的整合，从而导致损伤中心处受损脊髓组织的组织学修复。消融后由于组织修复，有效地防止了神经胶质瘢痕的形成。此外，在损伤中心 P0（髓鞘糖蛋白 P-zero）阳性，由已知能髓鞘再生和脱髓鞘轴突的施万细胞形成的髓鞘形成明显增加，与对照组相比数量明显增加。然而，用 BBB 旷场量表评估时，本研究中未观察到运动功能的显著改善；讨论了可能的原因。
