Karadeniz Technical University, Faculty of Sciences, Department of Biology, 61080 Trabzon, Turkey.
Yeditepe University, Faculty of Engineering and Architecture, Department of Genetics and Bioengineering, 34755 Istanbul, Turkey.
Int J Syst Evol Microbiol. 2012 Apr;62(Pt 4):849-855. doi: 10.1099/ijs.0.031914-0. Epub 2011 May 27.
Two Gram-positive, moderately thermophilic, endospore-forming, rod-shaped, motile bacteria, designated PDF25T and PDF30, were isolated from Karakoc hot spring in the province of Izmir, Turkey, and were characterized in order to determine their phylogenetic positions. 16S rRNA gene sequence analysis revealed that the two strains belonged to the genus Brevibacillus; strain PDF25T showed highest sequence similarity to strain PDF30 (99.4 %) and Brevibacillus thermoruber DSM 7064T (98.5 %). The major fatty acids of strain PDF25T were iso-C15:0 (39.30 %), anteiso-C15:0 (26.10 %) and iso-C16:0 (14.75 %). Polar lipid analysis revealed diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, and a variety of unidentified aminophospholipids, phospholipids and aminolipids. The major isoprenoid quinone was MK-7. The G+C content of the genomic DNA was 56.09 mol%. DNA-DNA hybridization experiments revealed 58 % relatedness between strain PDF25T and B. thermoruber DSM 7064T. Based on these data, the two strains are considered to represent a novel species of the genus Brevibacillus, for which the name Brevibacillus aydinogluensis sp. nov. is proposed. The type strain is PDF25T (=DSM 24395T=LMG 26289T).
两株革兰氏阳性、中度嗜热、产芽孢、杆状、运动的细菌，分别命名为 PDF25T 和 PDF30，从土耳其伊兹密尔省的卡拉科奇温泉中分离得到，并对其进行了特征描述，以确定它们的系统发育地位。16S rRNA 基因序列分析表明，这两株菌属于短芽孢杆菌属；菌株 PDF25T 与菌株 PDF30（99.4%）和 Brevibacillus thermoruber DSM 7064T（98.5%）的序列相似性最高。菌株 PDF25T 的主要脂肪酸为 iso-C15:0（39.30%）、anteiso-C15:0（26.10%）和 iso-C16:0（14.75%）。极性脂分析表明存在二磷脂酰甘油、磷脂酰甘油、磷脂单甲基乙醇胺、磷脂乙醇胺和多种未鉴定的胺磷脂、磷脂和胺脂。主要的异戊烯醌为 MK-7。基因组 DNA 的 G+C 含量为 56.09 mol%。DNA-DNA 杂交实验表明菌株 PDF25T 与 B. thermoruber DSM 7064T 之间的相关性为 58%。基于这些数据，这两株菌被认为代表了短芽孢杆菌属的一个新种，命名为 Brevibacillus aydinogluensis sp. nov.。模式菌株为 PDF25T（=DSM 24395T=LMG 26289T）。
