College of Fisheries, Henan Normal University, Xinxiang, 453007, PR China.
Synthetic Biology Engineering Lab of Henan Province, College of Life Sciences and Technology, Xinxiang Medical University, Xinxiang 453003, PR China.
Int J Syst Evol Microbiol. 2020 Nov;70(11):5693-5700. doi: 10.1099/ijsem.0.004462. Epub 2020 Sep 15.
Strain CFH S0501, a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella, was isolated from a sediment sample collected from the Yellow River in Henan Province, PR China. Optimum growth was observed at 28 °C, pH 7.0 and without NaCl. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain belonged to the genus and was closely related to DSM 8445 and Gsoil 3088 (with 96.8 and 96.7 % sequence similarity, respectively). The predominant menaquinone was MK-7. Major cellular fatty acids were anteiso-C and iso-C. Polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, two unidentified phospholipids and an unidentified polar lipid. The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid. The genome size was 5.26 Mbp with a G+C content of 49.7 mol%. The average nucleotide identity (ANI) and DNA-DNAhybridization (DDH) values between CFH S0501 and the other species of the genus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %). Based on physiological properties, chemotaxonomic characteristics and low ANI and DDH results, strain CFH S0501 is considered to represent a novel species, for which the name sp. nov. is proposed. The type strain is CFH S0501 (=DSM 29940=BCRC 80809).
CFH S0501 菌株,是一种新型革兰氏阳性、需氧、杆状、能形成内生孢子并具有周生鞭毛的运动微生物,从中国河南省黄河沉积物样本中分离得到。最适生长温度为 28°C,pH 值为 7.0,不需要氯化钠。基于 16S rRNA 基因序列的系统发育分析表明,该菌株属于 属,与 DSM 8445 和 Gsoil 3088 (分别具有 96.8%和 96.7%的序列相似性)最为密切相关。主要的膜烯醌为 MK-7。主要的细胞脂肪酸为 anteiso-C 和 iso-C。极性脂由双磷脂酰甘油、磷脂酰甘油、磷脂酰单甲基乙醇胺、磷脂酰乙醇胺、两种未鉴定的磷脂和一种未鉴定的极性脂组成。细胞壁肽聚糖中含有 meso-二氨基庚二酸。基因组大小为 5.26 Mbp,G+C 含量为 49.7 mol%。CFH S0501 与 属中其他种之间的平均核苷酸同一性 (ANI) 和 DNA-DNA 杂交 (DDH) 值较低(ANI<86.11%,ANb<70.30%和 DDH<25.00%)。根据生理特性、化学分类特征以及低 ANI 和 DDH 结果,菌株 CFH S0501 代表一种新型物种,提议将其命名为 sp. nov.。其模式菌株为 CFH S0501(=DSM 29940=BCRC 80809)。