Khan Inam Ullah, Habib Neeli, Asem Mipeshwaree Devi, Salam Nimaichand, Xiao Min, Zhou En-Min, Zhi Xiao-Yang, Li Wen-Jun
1Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming, 650091, PR China.
2Department of Biological Sciences, Gomal University, Dera Ismail Khan, KPK, Pakistan.
Int J Syst Evol Microbiol. 2019 Feb;69(2):337-342. doi: 10.1099/ijsem.0.003103. Epub 2018 Nov 7.
A Gram-stain-negative, aerobic, non-spore-forming and rod-shaped bacterium, designated YIM 730274, was isolated from a sediment sample collected from a hot spring located in Tibet, PR China, and was characterized by using a polyphasic taxonomy approach. Cells were motile by means of a polar flagellum. The strain was oxidase- and catalase-positive, and contained polyalkanoates and polyphosphate as storage polymers. Growth occurred at 25-50 °C, at pH 6.0-8.5 and with 0.5-1.0 % NaCl. The major fatty acids (>10 %) were summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C16 : 0. The known polar lipids comprised of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylserine. The isoprenoid quinone was Q-8. The G+C content of genomic DNA was 70.7 mol%. The results of phylogenetic analyses based on 16S rRNA gene sequences indicated that the strain forms a monophyletic branch at the periphery of the evolutionary radiation occupied by the genus Aquabacterium in the class Betaproteobacteria. The most closely related phylogenetic neighbours were Aquabacterium limnoticumABP-4 (97.8 % 16S rRNA gene sequence identity) and Aquabacterium communeB8 (97.2 % 16SrRNA gene sequence identity). DNA-DNA relatedness values between YIM 730274 and A. limnoticum KCTC 23306 (46.4±0.4 %) and A. commune DSM 11901 (42.2±1.2 %) were well below the 70 % limit for species identification. YIM 730274 was distinguishable from other members of the genus Aquabacterium by the differences in phenotypic, chemotaxonomic and genotypic characteristics. YIM 730274 merits recognition as a representative of a novel species of the genus Aquabacterium. It is proposed that the isolate should be classified in the genus Aquabacterium as representing a novel species, Aquabacteriumtepidiphilum sp. nov. The type strain is YIM 730274 (=KCTC 52716=CCTCC AB 2016295).
从中国西藏一处温泉采集的沉积物样本中分离出一株革兰氏阴性、需氧、无芽孢形成的杆状细菌，命名为YIM 730274，并采用多相分类法对其进行了特征描述。细胞借助极生鞭毛运动。该菌株氧化酶和过氧化氢酶呈阳性，含有聚羟基脂肪酸酯和聚磷酸盐作为储存聚合物。在25 - 50 °C、pH 6.0 - 8.5和0.5 - 1.0 % NaCl条件下可生长。主要脂肪酸（>10 %）为特征性总和3（C16 : 1ω6c和/或C16 : 1ω7c）、特征性总和8（C18 : 1ω7c和/或C18 : 1ω6c）和C16 : 0。已知极性脂类由双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油和磷脂酰丝氨酸组成。类异戊二烯醌为Q - 8。基因组DNA的G + C含量为70.7 mol%。基于16S rRNA基因序列的系统发育分析结果表明，该菌株在β-变形菌纲水生杆菌属所占据的进化辐射边缘形成一个单系分支。最密切相关的系统发育近缘种是水生嗜冷杆菌ABP - 4（16S rRNA基因序列同一性为97.8 %）和水生杆菌B8（16S rRNA基因序列同一性为97.2 %）。YIM 730274与嗜冷水生杆菌KCTC 23306（46.4±0.4 %）和水生杆菌DSM 11901（42.2±1.2 %）之间的DNA - DNA相关性值远低于物种鉴定的70 %界限。YIM 730274在表型、化学分类和基因型特征方面与水生杆菌属的其他成员不同。YIM 730274应被视为水生杆菌属一个新物种的代表。建议将该分离株归类于水生杆菌属，代表一个新物种，嗜温水生杆菌新种。模式菌株为YIM 730274（=KCTC 52716=CCTCC AB 2016295）。
