Departamento de Produção Animal, Faculdade de Medicina Veterinária e Zootecnia-UNESP, Botucatu-SP 18618-000, Brazil.
J Anim Sci. 2011 Oct;89(10):3116-24. doi: 10.2527/jas.2011-3909. Epub 2011 May 27.
Five experiments evaluated the effects of supplemental Ca salts of PUFA on reproductive function of Bos indicus beef cows. In Exp. 1, nonlactating and multiparous grazing cows (n = 51) were assigned to receive (as-fed basis) 0.1 kg of a protein-mineral mix + 0.1 kg of ground corn per cow/d, in addition to 0.1 kg per cow/d of 1) Ca salts of PUFA (PF), 2) Ca salts of SFA (SF), or 3) kaolin (control). Treatments were offered from d 0 to 20 of the estrous cycle. No treatment effects were detected on serum progesterone concentrations (P = 0.83), day of luteolysis (P = 0.86), or incidence of short cycles (P = 0.84). In Exp. 2, nonlactating and multiparous grazing cows (n = 43) were assigned to receive PF, SF, or control from d 0 to 8 of the estrous cycle. On d 6, all cows received (intramuscularly) 25 mg of PGF(2α). No treatment effects were detected on serum progesterone concentrations on d 6 (P = 0.37), and incidence (P = 0.67) or estimated time of luteolysis (P = 0.44). In Exp. 3, twenty-seven lactating and multiparous grazing cows, approximately 30 to 40 d postpartum, were assigned to receive PF or control for 10 d beginning at the first postpartum ovulation. No treatment effects were detected (P = 0.85) on incidence of short cycles. In Exp. 4, lactating and multiparous grazing cows (n = 1,454), approximately 40 to 60 d postpartum, were assigned to receive 1 of the 7 treatments for 28 d after timed AI (TAI; d 0): 1) control from d 0 to 28, 2) SF from d 0 to 14 and then control, 3) PF from d 0 to 14 and then control, 4) SF from d 0 to 21 and then control, 5) PF from d 0 to 21 and then control, 6) SF from d 0 to 28, and 7) PF from d 0 to 28. Cows receiving PF for more than 21 d after TAI had greater (P < 0.01) pregnancy to TAI compared with all other treatments combined (50.4 vs. 42.4%, respectively). In Exp. 5, lactating and multiparous grazing cows (n = 501), approximately 40 to 60 d postpartum, were assigned to receive 1 of the 4 treatments for 21 d after TAI (d 0): 1) PF from d 0 to 14 and then control, 2) control from d 0 to 6 and then PF, 3) control from d 0 to 13 and then PF, and 4) PF from d 0 to 21. Cows receiving PF after d 14 of the experiment had greater (P = 0.02) pregnancy to TAI compared with cows not receiving PF during the same period (46.8 vs. 33.1%, respectively). In summary, supplemental Ca salts of PUFA during the expected time of luteolysis increased pregnancy to TAI in beef cows.
五项实验评估了 PUFA 的钙盐补充剂对印度瘤牛种肉牛繁殖功能的影响。在实验 1 中，非泌乳和经产放牧牛（n = 51）被分配为每天每头牛额外接受 0.1 公斤蛋白质-矿物质混合物+0.1 公斤粉碎玉米，除了每天每头牛还接受 1）PUFA 的钙盐（PF），2）SFA 的钙盐（SF），或 3）高岭土（对照）。处理从发情周期的第 0 天到第 20 天提供。血清孕激素浓度（P = 0.83）、黄体溶解日（P = 0.86）或短周期发生率（P = 0.84）均未受到处理影响。在实验 2 中，非泌乳和经产放牧牛（n = 43）从发情周期的第 0 天到第 8 天接受 PF、SF 或对照。在第 6 天，所有牛都接受了（肌肉内）25 毫克 PGF（2α）。第 6 天血清孕激素浓度无处理影响（P = 0.37），且短周期发生率（P = 0.67）或黄体溶解估计时间（P = 0.44）无处理影响。在实验 3 中，27 头泌乳和经产放牧牛，大约产后 30 到 40 天，从第一次产后排卵开始接受 PF 或对照治疗 10 天。短周期发生率无处理影响（P = 0.85）。在实验 4 中，大约产后 40 到 60 天的泌乳和经产放牧牛（n = 1,454）在定时人工授精（TAI；第 0 天）后接受 7 种处理中的 1 种 28 天：1）从第 0 天到第 28 天的对照，2）从第 0 天到第 14 天的 SF 然后是对照，3）从第 0 天到第 14 天的 PF 然后是对照，4）从第 0 天到第 21 天的 SF 然后是对照，5）从第 0 天到第 21 天的 PF 然后是对照，6）从第 0 天到第 28 天的 SF，和 7）从第 0 天到第 28 天的 PF。TAI 后接受 PF 治疗超过 21 天的牛与所有其他治疗组合相比（分别为 50.4%和 42.4%）具有更高的怀孕率（P < 0.01）。在实验 5 中，大约产后 40 到 60 天的泌乳和经产放牧牛（n = 501）在 TAI 后接受 4 种处理中的 1 种 21 天：1）从第 0 天到第 14 天的 PF 然后是对照，2）从第 0 天到第 6 天的对照然后是 PF，3）从第 0 天到第 13 天的对照然后是 PF，和 4）从第 0 天到第 21 天的 PF。在实验的第 14 天之后接受 PF 的牛与同一时期未接受 PF 的牛相比（分别为 46.8%和 33.1%），具有更高的 TAI 怀孕率（P = 0.02）。总之，在黄体溶解预期时间补充 PUFA 的钙盐增加了肉牛的 TAI 怀孕率。
