Cooke R F, Cappellozza B I, Guarnieri Filho T A, Depner C M, Lytle K A, Jump D B, Bohnert D W, Cerri R L A, Vasconcelos J L M
Oregon State University- Eastern Oregon Agricultural Research Center, Burns 97720.
J Anim Sci. 2014 May;92(5):2239-50. doi: 10.2527/jas.2013-7422. Epub 2014 Mar 26.
The objective of this experiment was to compare fatty acid (FA) concentrations in plasma and reproductive tissues as well as hormones and expression of genes associated with pregnancy establishment in beef cows supplemented or not with Ca salts of soybean oil (CSSO) beginning after timed AI. Ninety nonlactating multiparous Nelore (Bos indicus) cows were timed inseminated on d 0 of the experiment and divided into 18 groups of 5 cows/group. Groups were randomly assigned to receive (as-fed basis) 100 g of a protein-mineral mix plus 100 g of ground corn per cow daily in addition to 1) 100 g/cow daily of CSSO (n = 9) or 2) 100 g/cow daily of kaolin (CON; rumen-inert indigestible substance; n = 9). All groups were maintained in a single Brachiaria brizanta pasture (24 ha) with ad libitum access to forage and water. However, groups were segregated daily and offered treatments individually at the working facility during the experimental period (d 0 to 18). Blood samples were collected and transrectal ultrasonography was performed to verify ovulation and estimate corpus luteum (CL) volume immediately before AI (d 0) and on d 7 and 18 of the experiment. On d 19, 36 cows (18 cows/treatment; 2 cows/group) diagnosed without the presence of a CL on d 0 but with a CL greater than 0.38 cm(3) in volume on d 7 and 18 were slaughtered for collection of conceptus, uterine luminal flushing, and tissue samples from the CL and endometrium. Cows receiving CSSO had greater concentrations of linoleic and other ω-6 FA in plasma (P < 0.01), endometrium (P ≤ 0.05), CL (P ≤ 0.05), and conceptus (P ≤ 0.08) compared to CON. On d 7 of the experiment, CSSO-supplemented cows had greater plasma progesterone concentrations (P < 0.01) and CL volume (P = 0.02) compared to CON, whereas no treatment effects were detected (P ≥ 0.15) for these parameters on d 18 (treatment × day interaction; P < 0.01). Cows receiving CSSO tended (P = 0.09) to have greater concentrations of interferon-tau in the uterine flushing media compared with CON. However, no treatment effects were detected for mRNA expression genes associated with pregnancy establishment in endometrial, CL, and conceptus samples (P ≥ 0.12). In summary, supplementing beef cows with 100 g of CSSO beginning after AI favored incorporation of ω-6 FA into their circulation, reproductive tissues, and conceptus, without impacting expression of genes associated with pregnancy establishment on d 19 of gestation.
本实验的目的是比较在定时人工授精（AI）后开始补充或不补充大豆油钙盐（CSSO）的肉牛血浆和生殖组织中的脂肪酸（FA）浓度，以及与妊娠建立相关的激素和基因表达。90头非泌乳经产内罗牛（印度瘤牛）在实验第0天进行定时输精，并分为18组，每组5头。各组随机分配接受（以饲喂量计）每头母牛每天100 g蛋白质 - 矿物质混合物加100 g粉碎玉米，此外，1）每头母牛每天100 g CSSO（n = 9）或2）每头母牛每天100 g高岭土（CON；瘤胃惰性不可消化物质；n = 9）。所有组均饲养在一片单一的臂形草（24公顷）牧场中，可自由采食草料和饮水。然而，在实验期间（第0天至第18天），各组每天分开，并在工作设施中单独给予处理。在AI前（第0天）以及实验第7天和第18天采集血样并进行经直肠超声检查以验证排卵并估计黄体（CL）体积。在第19天，对36头母牛（每个处理18头；每组2头）进行屠宰，这些母牛在第0天诊断为无CL，但在第7天和第18天CL体积大于0.38 cm³，用于采集胚胎、子宫腔冲洗液以及CL和子宫内膜的组织样本。与CON组相比，接受CSSO的母牛血浆、子宫内膜（P≤0.05）、CL（P≤0.05）和胚胎（P≤0.08）中的亚油酸和其他ω-6 FA浓度更高。在实验第7天，与CON组相比，补充CSSO的母牛血浆孕酮浓度更高（P < 0.01），CL体积更大（P = 0.02），而在第18天这些参数未检测到处理效应（P≥0.15）（处理×天数交互作用；P < 0.01）。与CON组相比，接受CSSO的母牛子宫冲洗液中干扰素 - τ浓度有升高趋势（P = 0.09）。然而，在子宫内膜、CL和胚胎样本中，未检测到与妊娠建立相关的mRNA表达基因存在处理效应（P≥0.12）。总之，AI后开始给肉牛补充100 g CSSO有利于ω-6 FA融入其循环、生殖组织和胚胎，且不影响妊娠第19天与妊娠建立相关基因的表达。
