Coordinación de Ciencia de los Alimentos, Centro de Investigación en Alimentación y Desarrollo A.C., Carretera a la Victoria Km. 0.6, Hermosillo, Sonora 83304, México.
J Anim Sci. 2011 Oct;89(10):3125-31. doi: 10.2527/jas.2010-2996. Epub 2011 May 27.
Adherence to the gastrointestinal tract is a key element desirable for many of the proposed beneficial health effects of probiotic bacteria. The aims of this study were to determine the amounts of adhesion of 3 Lactobacillus salivarius strains (Lb6, Lb9, and Lb10) to porcine small intestinal mucins and to determine whether adhesion is a function of lectin-like activities. Dot and Western blot assays were performed to investigate bacterial adhesion. Several carbohydrates and glycoproteins were evaluated to determine whether they interfered with adhesion of the Lactobacillus strains to intestinal mucins and to determine whether they had lectin-like activities. The Lb9 and Lb10 strains had greater association with piglet mucins than did those from 22- to 24-wk-old finishing pigs (P = 0.021 and 0.037, respectively), whereas the Lb6 strain adhered to both (P = 0.138). Western blot assays showed that bacterial adhesion detected piglet mucosa from the duodenum, jejunum, and ileum. In finishing pigs, the adhesion was variable throughout the gastrointestinal tract. Galactose and mannose diminished the interaction of the Lb9 and Lb10 strains in intestinal mucosa (P = 0.028 and 0.026, respectively), whereas pig gastric mucin reduced the adhesion of the Lb6 strain (P = 0.013). Adhesion of the Lb9 and Lb10 strains to intestinal mucosa was less after protease treatment (P = 0.023 and 0.018, respectively), which indicates that proteins are needed for the Lb9 and Lb10 strains to recognize mucin. The Lb6 strain also demonstrated diminished adhesion after periodate treatment (P = 0.038). From these results, we suggest that the nature of the bacterial lectin-like substance is a surface protein that loosely binds to the bacterial cell surface. All the tested strains adhered to specific targets in the small intestinal mucosa of piglets, and the bacteria had lectin-like proteins involved in this adhesion.
对许多拟议的益生菌有益健康影响，黏附于胃肠道是一个关键要素。本研究的目的是确定 3 株唾液乳杆菌（Lb6、Lb9 和 Lb10）黏附于猪小肠黏蛋白的量，并确定黏附是否是凝集素样活性的功能。通过点印迹和 Western blot 分析来研究细菌黏附。评估了几种碳水化合物和糖蛋白，以确定它们是否干扰了乳酸菌菌株对肠黏蛋白的黏附，并确定它们是否具有凝集素样活性。Lb9 和 Lb10 菌株与仔猪黏蛋白的关联大于 22-24 周龄育肥猪（P = 0.021 和 0.037），而 Lb6 菌株则与两者都有联系（P = 0.138）。Western blot 分析表明，细菌黏附可检测到十二指肠、空肠和回肠的仔猪黏膜。在育肥猪中，黏附在整个胃肠道中是可变的。半乳糖和甘露糖减少了 Lb9 和 Lb10 菌株与肠黏膜的相互作用（P = 0.028 和 0.026），而猪胃黏蛋白减少了 Lb6 菌株的黏附（P = 0.013）。Lb9 和 Lb10 菌株经蛋白酶处理后黏附于肠黏膜的能力降低（P = 0.023 和 0.018），这表明黏附需要蛋白质来识别黏蛋白。经过碘酸钠处理后，Lb6 菌株的黏附能力也降低（P = 0.038）。从这些结果中，我们认为细菌凝集素样物质的性质是一种表面蛋白，它松散地结合在细菌细胞表面。所有测试的菌株都黏附于仔猪小肠黏膜的特定靶标，并且细菌具有参与这种黏附的凝集素样蛋白。
