College of Agriculture, University of Kentucky, Room no. 434, Plant Science Building 1405, Veterans drive, Lexington, KY 40546-0312, USA.
J Nat Med. 2012 Jan;66(1):1-7. doi: 10.1007/s11418-011-0541-y. Epub 2011 May 29.
This study reports an appreciable yield of podophyllotoxin (PDT) in P. hexandrum plants grown ex situ under polyhouse conditions of a temperate locale. The PDT content of below-ground parts was affected by both plant age and growth period. However, only the effect of plant age on PDT content was significant. Thus, the highest amounts of PDT were recorded in the below-ground parts of 2-year-old plants harvested during the late-growth period (LGP). High total soluble sugars in the below-ground parts during the early growth period (EGP) and the highest nitrate and nitrate reductase in the leaves of 2-year-old plants during the peak-growth period (PGP) indicated higher mobilization and assimilation of starch and nitrate. Probably the surplus carbon and nitrogen gained during the PGP were diverted from aerial parts to below-ground parts during the LGP and in turn contributed to the synthesis of higher amounts of PDT. This study shows that commercial cultivation of P. hexandrum is possible under ex situ temperate conditions.
本研究报告了在温带地区温室条件下生长的鬼臼（P. hexandrum）植物中可观的鬼臼毒素（PDT）产量。地下部分的 PDT 含量受植物年龄和生长周期的影响。然而，只有植物年龄对 PDT 含量的影响是显著的。因此，在生长后期（LGP）收获的 2 年生植物的地下部分中记录到了最高量的 PDT。在早期生长阶段（EGP）地下部分中总可溶性糖含量较高，在高峰期生长阶段（PGP）2 年生植物叶片中的硝酸盐和硝酸还原酶含量最高，表明淀粉和硝酸盐的动员和同化较高。可能在 PGP 期间获得的多余碳和氮在 LGP 期间从地上部分转移到地下部分，并反过来有助于合成更高量的 PDT。这项研究表明，在温带地区的异地条件下，鬼臼的商业种植是可能的。
