Minafra S, Morello V, Glorioso F, La Fiura A M, Tomasino R M, Feo S, McIntosh D, Woolley D E
Istituto di Istologia ed Embriologia, Università di Palermo, Italy.
Br J Cancer. 1989 Aug;60(2):185-92. doi: 10.1038/bjc.1989.248.
A cell line, designated 8701-BC, was established in culture from tissue fragments of primary ductal infiltrating carcinoma of human breast. The cell cultures after the sixth passage were devoid of contaminating fibroblasts as judged by the positive staining of all cells with the specific epithelial cell markers carcinoembryonic antigen (CEA), tissue polypeptide antigen (TPA) and cytokeratin 8. The epithelial nature of these cells was confirmed by ultrastructural analyses which demonstrated the retention of specific structural properties characteristic of the original tumour. The cells possessed an abnormal karyotype with 55-60 chromosomes per cell with numerous rearrangements. They do not express HLA antigens and the c-myc gene was not amplified. The 8701-BC cells have a doubling time of approx. 29h and have been maintained in culture for more than 100 passages. These properties suggest the establishment of a human neoplastic cell line which, with its ability to produce homotrimer collagen in vitro, will provide a useful model system for the study of tumour cell:stromal matrix interactions.
一株命名为8701-BC的细胞系由人乳腺原发性导管浸润癌的组织碎片培养建立。通过用特异性上皮细胞标志物癌胚抗原（CEA）、组织多肽抗原（TPA）和细胞角蛋白8对所有细胞进行阳性染色判断，第六代后的细胞培养物中没有污染的成纤维细胞。这些细胞的上皮性质通过超微结构分析得到证实，该分析表明保留了原始肿瘤特有的特定结构特性。这些细胞具有异常核型，每个细胞有55 - 60条染色体，且有许多重排。它们不表达HLA抗原，c-myc基因也未扩增。8701-BC细胞的倍增时间约为29小时，已在培养中维持了100多代。这些特性表明建立了一种人肿瘤细胞系，其在体外产生同源三聚体胶原蛋白的能力将为研究肿瘤细胞与基质相互作用提供一个有用的模型系统。
