Laboratory of Organic Chemistry, Wageningen University and Research Center, Dreijenplein 8, 6703 HB, Wageningen, The Netherlands.
Langmuir. 2011 Jul 5;27(13):8126-33. doi: 10.1021/la200932w. Epub 2011 Jun 1.
The preparation and characterization of self-assembled monolayers on copper with n-alkyl and functional thiols was investigated. Well-ordered monolayers were obtained, while the copper remained oxide-free. Direct attachment of N-succinimidyl mercaptoundecanoate (NHS-MUA) onto the copper surface allowed for the successful attachment of biomolecules, such as β-d-glucosamine, the tripeptide glutathione, and biotin. Notably, the copper surfaces remained oxide-free even after two reaction steps. All monolayers were characterized by static water contact angle measurements, X-ray photoelectron spectroscopy, and infrared reflection absorption spectroscopy. In addition, the biotinylated copper surfaces were employed in the immobilization of biomolecules such as streptavidin.
研究了具有 n-烷基和官能团硫醇的自组装单层在铜上的制备和特性。得到了有序的单层，而铜仍保持无氧化物状态。N-琥珀酰亚胺基巯基十一酸酯（NHS-MUA）直接附着在铜表面上，允许成功地附着生物分子，如β-D-葡萄糖胺、三肽谷胱甘肽和生物素。值得注意的是，即使经过两个反应步骤，铜表面仍保持无氧化物状态。所有的单层都通过静态水接触角测量、X 射线光电子能谱和红外反射吸收光谱进行了表征。此外，生物素化的铜表面被用于固定生物分子，如链霉亲和素。
