Feldman D, Sherin J E, Press W A, Bear M F
Center for Neural Science, Brown University, Providence, RI 02912.
Exp Brain Res. 1990;80(2):252-9. doi: 10.1007/BF00228153.
As a functional measure of NMDA receptor effectiveness in kitten striate cortex, the uptake of 45Ca by visual cortical slices was measured after 2 minute bath applications of N-methyl-D-aspartate (NMDA). Significant Ca uptake occurred in response to 12.5-100 microM NMDA in slices prepared from visual cortex of normal animals aged 28-48 days. Basal uptake (in the absence of NMDA) was increased and evoked uptake was decreased in visual cortical slices prepared from age-matched dark-reared animals. Four days of binocular deprivation in otherwise normally reared animals had no effect on basal uptake, but significantly lowered NMDA-evoked Ca uptake at agonist concentrations greater than 25 microM. These data suggest that even brief manipulations of sensory experience are sufficient to alter visual cortical calcium regulation.
作为小猫视皮层中NMDA受体效能的一项功能指标，在应用N-甲基-D-天冬氨酸（NMDA）进行2分钟浸浴处理后，测定了视皮层切片对45Ca的摄取情况。在28至48日龄正常动物视皮层制备的切片中，12.5 - 100微摩尔的NMDA可引起显著的钙摄取。在年龄匹配的暗饲养动物视皮层制备的切片中，基础摄取（无NMDA时）增加，而诱发摄取减少。在其他方面正常饲养的动物中，4天的双眼剥夺对基础摄取无影响，但在激动剂浓度大于25微摩尔时，显著降低了NMDA诱发的钙摄取。这些数据表明，即使是对感觉经验的短暂操纵也足以改变视皮层的钙调节。
