将 EBV 永生化 B 淋巴细胞细胞系重编程为诱导多能干细胞。
Reprogramming of EBV-immortalized B-lymphocyte cell lines into induced pluripotent stem cells.
机构信息
Stem Cell Biology Laboratory, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA.
出版信息
Blood. 2011 Aug 18;118(7):1801-5. doi: 10.1182/blood-2011-03-340620. Epub 2011 May 31.
Abstract
EBV-immortalized B lymphocyte cell lines have been widely banked for studying a variety of diseases, including rare genetic disorders. These cell lines represent an important resource for disease modeling with the induced pluripotent stem cell (iPSC) technology. Here we report the generation of iPSCs from EBV-immortalized B-cell lines derived from multiple inherited disease patients via a nonviral method. The reprogramming method for the EBV cell lines involves a distinct protocol compared with that of patient fibroblasts. The B-cell line-derived iPSCs expressed pluripotency markers, retained the inherited mutation and the parental V(D)J rearrangement profile, and differentiated into all 3 germ layer cell types. There was no integration of the reprogramming-related transgenes or the EBV-associated genes in these iPSCs. The ability to reprogram the widely banked patient B-cell lines will offer an unprecedented opportunity to generate human disease models and provide novel drug therapies.
摘要
EBV 永生化 B 淋巴细胞细胞系已被广泛储存，用于研究多种疾病，包括罕见的遗传疾病。这些细胞系代表了使用诱导多能干细胞（iPSC）技术进行疾病建模的重要资源。在这里，我们通过非病毒方法从多个遗传疾病患者的 EBV 永生化 B 细胞系中生成了 iPSC。与患者成纤维细胞相比，该 EBV 细胞系的重编程方法涉及独特的方案。B 细胞系衍生的 iPSC 表达多能性标记物，保留了遗传突变和亲本 V(D)J 重排谱，并分化为所有 3 个胚层细胞类型。这些 iPSC 中没有重编程相关转基因或 EBV 相关基因的整合。重编程广泛储存的患者 B 细胞系的能力将为生成人类疾病模型和提供新型药物治疗提供前所未有的机会。
相似文献
1
Reprogramming of EBV-immortalized B-lymphocyte cell lines into induced pluripotent stem cells.将 EBV 永生化 B 淋巴细胞细胞系重编程为诱导多能干细胞。
Blood. 2011 Aug 18;118(7):1801-5. doi: 10.1182/blood-2011-03-340620. Epub 2011 May 31.
2
Human lymphoblastoid B-cell lines reprogrammed to EBV-free induced pluripotent stem cells.人淋巴母细胞系重编程为 EBV 无诱导多能干细胞。
Blood. 2011 Aug 18;118(7):1797-800. doi: 10.1182/blood-2011-01-332064. Epub 2011 Jun 27.
3
Hematopoietic cells as sources for patient-specific iPSCs and disease modeling.造血细胞作为患者特异性 iPSCs 和疾病建模的来源。
Cell Cycle. 2011 Sep 1;10(17):2840-4. doi: 10.4161/cc.10.17.17180.
4
Reliable generation of induced pluripotent stem cells from human lymphoblastoid cell lines.从人淋巴母细胞系可靠地生成诱导多能干细胞。
Stem Cells Transl Med. 2014 Dec;3(12):1429-34. doi: 10.5966/sctm.2014-0121. Epub 2014 Oct 8.
5
Reprogramming human B cells into induced pluripotent stem cells and its enhancement by C/EBPα.将人 B 细胞重编程为诱导多能干细胞及其通过 C/EBPα 的增强作用。
Leukemia. 2016 Mar;30(3):674-82. doi: 10.1038/leu.2015.294. Epub 2015 Oct 26.
6
Modeling neurological diseases with induced pluripotent cells reprogrammed from immortalized lymphoblastoid cell lines.利用从永生化淋巴母细胞系重编程而来的诱导多能细胞对神经疾病进行建模。
Mol Brain. 2016 Oct 3;9(1):88. doi: 10.1186/s13041-016-0267-6.
7
Reactivation of Endogenous Genes and Epigenetic Remodeling Are Barriers for Generating Transgene-Free Induced Pluripotent Stem Cells in Pig.内源性基因的重新激活和表观遗传重塑是在猪中生成无转基因诱导多能干细胞的障碍。
PLoS One. 2016 Jun 23;11(6):e0158046. doi: 10.1371/journal.pone.0158046. eCollection 2016.
8
Generation of transgene-free mouse induced pluripotent stem cells using an excisable lentiviral system.利用可切除的慢病毒系统生成无转基因的小鼠诱导多能干细胞。
Exp Cell Res. 2014 Apr 1;322(2):335-44. doi: 10.1016/j.yexcr.2014.02.006. Epub 2014 Feb 18.
9
Generation of Induced Pluripotent Stem Cells (iPSCs) from Adult Canine Fibroblasts.从成年犬成纤维细胞生成诱导多能干细胞（iPSC）
Methods Mol Biol. 2015;1330:69-78. doi: 10.1007/978-1-4939-2848-4_7.
10
mRNA Reprogramming of T8993G Leigh's Syndrome Fibroblast Cells to Create Induced Pluripotent Stem Cell Models for Mitochondrial Disorders.T8993G Leigh 综合征成纤维细胞的 mRNA 重编程，用于创建线粒体疾病的诱导多能干细胞模型。
Stem Cells Dev. 2019 Jul 1;28(13):846-859. doi: 10.1089/scd.2019.0045. Epub 2019 May 20.
引用本文的文献
1
Reliable multiplex generation of pooled induced pluripotent stem cells.可靠的诱导多能干细胞池的多重生成。
Cell Rep Methods. 2023 Sep 25;3(9):100570. doi: 10.1016/j.crmeth.2023.100570. Epub 2023 Aug 31.
2
Generating hematopoietic cells from human pluripotent stem cells: approaches, progress and challenges.从人类多能干细胞生成造血细胞：方法、进展与挑战。
Cell Regen. 2023 Sep 1;12(1):31. doi: 10.1186/s13619-023-00175-6.
3
An Overview on Promising Somatic Cell Sources Utilized for the Efficient Generation of Induced Pluripotent Stem Cells.用于高效诱导多能干细胞生成的有前景的体细胞来源概述
Stem Cell Rev Rep. 2021 Dec;17(6):1954-1974. doi: 10.1007/s12015-021-10200-3. Epub 2021 Jun 7.
4
A simplified approach for derivation of induced pluripotent stem cells from Epstein-Barr virus immortalized B-lymphoblastoid cell lines.一种从爱泼斯坦-巴尔病毒永生化B淋巴母细胞系中诱导多能干细胞的简化方法。
Heliyon. 2021 Apr 3;7(4):e06617. doi: 10.1016/j.heliyon.2021.e06617. eCollection 2021 Apr.
5
Convergence of human pluripotent stem cell, organoid, and genome editing technologies.人类多能干细胞、类器官和基因组编辑技术的融合。
Exp Biol Med (Maywood). 2021 Apr;246(7):861-875. doi: 10.1177/1535370220985808. Epub 2021 Jan 19.
6
A comparison of hepato-cellular in vitro platforms to study CYP3A4 induction.肝细胞体外模型比较用于研究 CYP3A4 诱导。
PLoS One. 2020 Feb 27;15(2):e0229106. doi: 10.1371/journal.pone.0229106. eCollection 2020.
7
Highly efficient induced pluripotent stem cell reprogramming of cryopreserved lymphoblastoid cell lines.冷冻保存的淋巴母细胞系的高效诱导多能干细胞重编程
J Biol Methods. 2020 Jan 8;7(1):e124. doi: 10.14440/jbm.2020.296. eCollection 2020.
8
Episomal Induced Pluripotent Stem Cells: Functional and Potential Therapeutic Applications.类胚胎干细胞：功能与潜在的治疗应用。
Cell Transplant. 2019 Dec;28(1_suppl):112S-131S. doi: 10.1177/0963689719886534. Epub 2019 Nov 14.
9
microRNA and mRNA interactions in induced pluripotent stem cell reprogramming of lymphoblastoid cell lines.微小RNA与信使核糖核酸在淋巴母细胞系诱导多能干细胞重编程中的相互作用
Am J Stem Cells. 2019 Aug 15;8(2):28-37. eCollection 2019.
10
Human-relevant preclinical in vitro models for studying hepatobiliary development and liver diseases using induced pluripotent stem cells.使用诱导多能干细胞研究肝胆发育和肝脏疾病的人类相关临床前体外模型。
Exp Biol Med (Maywood). 2019 May;244(8):702-708. doi: 10.1177/1535370219834895. Epub 2019 Feb 26.
本文引用的文献
1
In vivo liver regeneration potential of human induced pluripotent stem cells from diverse origins.不同来源的人诱导多能干细胞的体内肝脏再生潜力。
Sci Transl Med. 2011 May 11;3(82):82ra39. doi: 10.1126/scitranslmed.3002376.
2
Efficient generation of transgene-free induced pluripotent stem cells from normal and neoplastic bone marrow and cord blood mononuclear cells.高效地从正常和肿瘤性骨髓及脐血单个核细胞中生成无转基因的诱导多能干细胞。
Blood. 2011 Apr 7;117(14):e109-19. doi: 10.1182/blood-2010-07-298331. Epub 2011 Feb 4.
3
Simple generation of human induced pluripotent stem cells using poly-beta-amino esters as the non-viral gene delivery system.利用多聚-β-氨基酯作为非病毒基因传递系统来简单地生成人类诱导多能干细胞。
J Biol Chem. 2011 Apr 8;286(14):12417-28. doi: 10.1074/jbc.M110.168013. Epub 2011 Feb 1.
4
Efficient human iPS cell derivation by a non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures.通过具有独特表观遗传和基因表达特征的血细胞中的非整合质粒高效生成人诱导多能干细胞。
Cell Res. 2011 Mar;21(3):518-29. doi: 10.1038/cr.2011.12. Epub 2011 Jan 18.
5
Applications of patient-specific induced pluripotent stem cells; focused on disease modeling, drug screening and therapeutic potentials for liver disease.患者特异性诱导多能干细胞的应用；侧重于疾病建模、药物筛选和肝脏疾病的治疗潜力。
Int J Biol Sci. 2010 Dec 14;6(7):796-805. doi: 10.7150/ijbs.6.796.
6
Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA.利用合成修饰 mRNA 高效重编程人类细胞为多能性干细胞并进行定向分化。
Cell Stem Cell. 2010 Nov 5;7(5):618-30. doi: 10.1016/j.stem.2010.08.012. Epub 2010 Sep 30.
7
Reprogramming of human peripheral blood cells to induced pluripotent stem cells.人外周血细胞重编程为诱导多能干细胞。
Cell Stem Cell. 2010 Jul 2;7(1):20-4. doi: 10.1016/j.stem.2010.06.002.
8
Reprogramming of T cells from human peripheral blood.重编程人外周血中的T细胞。
Cell Stem Cell. 2010 Jul 2;7(1):15-9. doi: 10.1016/j.stem.2010.06.004.
9
Generation of induced pluripotent stem cells from human terminally differentiated circulating T cells.从人类终末分化的循环T细胞中诱导生成多能干细胞。
Cell Stem Cell. 2010 Jul 2;7(1):11-4. doi: 10.1016/j.stem.2010.06.003.
10
Derivation of induced pluripotent stem cells from human peripheral blood T lymphocytes.从人外周血 T 淋巴细胞中诱导多能干细胞的衍生。
PLoS One. 2010 Jun 29;5(6):e11373. doi: 10.1371/journal.pone.0011373.
Zotero 插件