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制备针对病毒蛋白的特异性抗血清的简单方法：针对马传染性贫血病毒蛋白p26和p16的兔抗血清。

Simple method for preparation of specific antisera against viral proteins: rabbit antisera against equine infectious anemia virus proteins p26 and p16.

作者信息

Barta V, Issel C J

出版信息

Am J Vet Res. 1978 Nov;39(11):1856-7.

PMID:216292
Abstract

A simple method for preparation of highly specific antisera against equine infectious anemia virus proteins p26 and p16 is described. Viral proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The motility of the viral proteins in the gel was compared with standards. Unstained portions of the slab gel were sliced into 5-mm bands, emulsified with Freund's complete adjuvant, and injected into rabbits to produce specific antisera.

摘要

描述了一种制备针对马传染性贫血病毒蛋白p26和p16的高特异性抗血清的简单方法。病毒蛋白通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳进行分离。将凝胶中病毒蛋白的迁移率与标准品进行比较。将平板凝胶的未染色部分切成5毫米宽的条带，与弗氏完全佐剂乳化后，注射到兔子体内以产生特异性抗血清。

相似文献

1
Simple method for preparation of specific antisera against viral proteins: rabbit antisera against equine infectious anemia virus proteins p26 and p16.制备针对病毒蛋白的特异性抗血清的简单方法：针对马传染性贫血病毒蛋白p26和p16的兔抗血清。
Am J Vet Res. 1978 Nov;39(11):1856-7.
2
Structural proteins of equine infectious anemia virus and their antigenic activity.马传染性贫血病毒的结构蛋白及其抗原活性。
Am J Vet Res. 1984 Jan;45(1):5-10.
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Enzyme-linked immunosorbent assay for detection of equine infectious anemia antibody to purified P26 viral protein.用于检测马传染性贫血病毒抗体对纯化P26病毒蛋白的酶联免疫吸附测定。
Am J Vet Res. 1984 Aug;45(8):1542-3.
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Immunoglobulin G subclass [IgG and IgG(T)] interaction with the P26 group specific antigen of equine infectious anemia virus: immunodiffusion and complement-fixation reactions.免疫球蛋白G亚类[IgG和IgG(T)]与马传染性贫血病毒P26群特异性抗原的相互作用：免疫扩散和补体结合反应。
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J Am Vet Med Assoc. 1982 Feb 1;180(3):276-8.

引用本文的文献

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Isolation and comparative biochemical properties of the major internal polypeptides of equine infectious anemia virus.马传染性贫血病毒主要内部多肽的分离及比较生化特性
J Virol. 1982 Jun;42(3):1029-38. doi: 10.1128/JVI.42.3.1029-1038.1982.