Nishimura M, Nakajima H
Am J Vet Res. 1984 Jan;45(1):5-10.
Using purified equine infectious anemia (EIA) virus labeled with 3H-glucosamine or 14C-protein hydrolysate, structural proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. As a result, 2 glycoproteins and 10 proteins with molecular weights (mol wt) ranging from 12,000 to 115,000 daltons were demonstrated. Of 12 structural proteins, 3 proteins, namely a glycoprotein with mol wt of 76,000 (gp76) and 2 proteins with mol wt of 25,000 (p25) and 12,000 (p12), respectively, had distinct antigenic activity from one another in immunodiffusion. Development of antibodies against gp76 and p25 was compared in infected horses. The antibody to gp76 appeared earlier and stronger than to p25 in horses infected with the homologous virus strain. The fraction with glycoproteins was found to have hemagglutinating activity which was inhibited by the serum sample from horses infected with equine infectious anemia virus.
使用用³H - 葡糖胺或¹⁴C - 蛋白质水解产物标记的纯化马传染性贫血（EIA）病毒，通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳分析结构蛋白。结果显示出2种糖蛋白和10种分子量在12,000至115,000道尔顿之间的蛋白质。在12种结构蛋白中，3种蛋白，即分子量为76,000的糖蛋白（gp76）和分子量分别为25,000（p25）和12,000（p12）的2种蛋白，在免疫扩散中彼此具有不同的抗原活性。比较了感染马中针对gp76和p25抗体的产生情况。在感染同源病毒株的马中，针对gp76的抗体出现得比针对p25的抗体更早且更强。发现含有糖蛋白的部分具有血凝活性，该活性被感染马传染性贫血病毒的马的血清样品所抑制。
