UMR 1233 INRA, VetAgro Sup, VetAgro Sup Campus Vétérinaire de Lyon 1 Avenue Bourgelat, 69280, Marcy L'Etoile, France.
Ecotoxicology. 2011 Aug;20(6):1432-41. doi: 10.1007/s10646-011-0700-7. Epub 2011 Jun 1.
The anti-vitamin Ks (AVKs) are widely used to control rodent populations. They inhibit Vitamin K regeneration by the Vitamin K Epoxide Reductase (VKOR) and cause a fatal hemorrhagic syndrome. Because of repeated use, some populations of commensal rodents have expressed resistance to these compounds. In Franche-Comté (France), the water vole exhibits cyclic population outbreaks. A second generation AVK, bromadiolone, has been used for the last 20 years to control vole populations. The aim of this study is to determine whether these repeated treatments could have led to the development of resistance to AVKs in water vole populations. We conducted enzymatic and genetic studies on water voles trapped in treated and non treated plot. The results indicate that voles from the most heavily treated area exhibit enzymatic changes in VKOR activity hence arguing for resistance to AVKs and that an intronic haplotype on the vkorc1 gene seems to be associated with these enzymatic changes.
抗维生素 K 药物（AVKs）被广泛用于控制啮齿动物种群。它们通过维生素 K 环氧化物还原酶（VKOR）抑制维生素 K 的再生，导致致命的出血综合征。由于反复使用，一些共生啮齿动物种群已经对这些化合物产生了抗性。在法国弗朗什孔泰（Franche-Comté），水鼠呈现周期性的种群爆发。第二代 AVK，溴敌隆，在过去的 20 年中被用于控制水鼠种群。本研究旨在确定这些反复处理是否导致水鼠种群对 AVKs 的抗性发展。我们对在处理和未处理的区域捕获的水鼠进行了酶学和遗传学研究。结果表明，来自受处理区的水鼠的 VKOR 活性存在酶学变化，这表明它们对 AVKs 有抗性，而 vkorc1 基因的内含子单倍型似乎与这些酶学变化有关。
