Oliveira Priscila Chagas, Pinheiro Antônio Luiz Barbosa, de Castro Isabele C V, Reis João Alves, Noia Manuela Pimentel, Gurgel Clarissa, Teixeira Cangussú Maria Cristina, Pedreira Ramalho Luciana Maria
Center of Biophotonics, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil.
Photomed Laser Surg. 2011 Sep;29(9):619-25. doi: 10.1089/pho.2010.2914. Epub 2011 Jun 1.
The study evaluated, by light microscopy, the repair process on third-degree burns on diabetic and nondiabetic rats, illuminated or not, with a polarized light (PL) source.
Burns are severe injuries that result in the loss of fluid and destruction of tissue, infection, and shock that may result in death. Diabetes mellitus is a public health problem that, being uncontrolled, causes severe disturbance to the body metabolism, including on wound healing. PL sources have been shown to be effective in improving healing in many situations.
Ninety male Wistar rats were divided into two groups (n=45): nondiabetic and diabetic. In one of the groups, diabetes mellitus was induced by streptozotocin. A third-degree burn, measuring 1.5×1.5 cm(2), was created in the dorsum of each animal. Phototherapy (λ400-2000 nm, 10.2 or 20.4 J/cm(2)) started immediately after burning and was repeated daily until animal death (7, 14, and 21 days). Specimens were taken, processed, and stained with H&E and Sirius red and immunomarked with cytokeratin (CK) AE1/AE3. Descriptive analysis was performed by light microscopy.
Animals subjected to phototherapy showed an acceleration of the repair, the dose of 10.2 J/cm(2) being the one that caused best results, including higher deposition of collagen, quicker inflammatory reaction, and improved revascularization.
Our results suggest that the use of PL (10.2 J/cm(2)) improves the healing of third-degree burns on both diabetic and nondiabetic animals.
本研究通过光学显微镜评估了偏振光源照射或未照射的糖尿病大鼠和非糖尿病大鼠三度烧伤的修复过程。
烧伤是严重的损伤，会导致体液流失、组织破坏、感染和休克，可能导致死亡。糖尿病是一个公共卫生问题，若未得到控制，会对身体代谢造成严重干扰，包括影响伤口愈合。已证明偏振光源在许多情况下能有效促进愈合。
90只雄性Wistar大鼠分为两组（每组n = 45）：非糖尿病组和糖尿病组。其中一组通过链脲佐菌素诱导糖尿病。在每只动物的背部造成1.5×1.5 cm²的三度烧伤。烧伤后立即开始光疗（波长400 - 2000 nm，10.2或20.4 J/cm²），并每天重复，直至动物死亡（7天、14天和21天）。采集标本，进行处理，用苏木精 - 伊红染色和天狼星红染色，并用细胞角蛋白（CK）AE1/AE3进行免疫标记。通过光学显微镜进行描述性分析。
接受光疗的动物显示修复加速，10.2 J/cm²的剂量产生了最佳效果，包括更高的胶原蛋白沉积、更快的炎症反应和改善的血管再生。
我们的结果表明，使用偏振光（10.2 J/cm²）可改善糖尿病和非糖尿病动物三度烧伤的愈合。
