Sayar Hamide, Gergerlioglu Nursadan, Seringec Nurten, Ozturk Perihan, Bulbuloglu Ertan, Karabay Gulten
Department of Pathology, Faculty of Medicine, Kahramanmaras Sutcu Imam University, Kahramanmaras, Turkey.
Department of Pathology, Faculty of Medicine, Selcuk University, Konya, Turkey.
Med Sci Monit Basic Res. 2014 Apr 3;20:36-46. doi: 10.12659/MSMBR.890337.
This experiment was performed to compare the effects of Phenytoin (PHT) and Hypericin (HP) cream on healing of burn wounds in rats.
Twenty rats were divided into 3 groups and second-degree burn wounds were created. The burn wounds in the first, second, and third groups were covered twice daily with PHT cream, HP cream, and saline (control), respectively. At the end of days 3, 7, 14, and 21, full-thickness skin biopsies were done for histopathologic and immunohistochemical analyses.
Histopathologic evaluations at the 14th day showed that re-epithelialization scores were greater in the HP group than the PHT group, but on day 21, re-epithelialization scores were higher in the PHT group than the HP group. Collagen content on days 3 and 14 in the PHT group was found to be higher than in the HP group. Well-vascularized granulation tissue on day 7 in the PHT group was higher than in other groups. HP and PHT groups had a significant increase in VEGF and TGF-b expression in burn wound healing area compared to the control group on all days.
Topical application of HP can promote re-epithelialization in burn wounds to shorten the wound healing time for superficial burns. Phenytoin, on the other hand, contributes to healing by increasing vascularized granulation tissue and collagen synthesis through re-epithelialization. The increased VEGF and TGF-b expression following PHT and HP treatment strongly indicate that PHT and HP treatment promotes VEGF and TGF-b production and action in the burn wound area.
本实验旨在比较苯妥英（PHT）和金丝桃素（HP）乳膏对大鼠烧伤创面愈合的影响。
将20只大鼠分为3组，制作二级烧伤创面。第一组、第二组和第三组的烧伤创面分别每日两次涂抹PHT乳膏、HP乳膏和生理盐水（对照组）。在第3、7、14和21天结束时，进行全层皮肤活检以进行组织病理学和免疫组织化学分析。
第14天的组织病理学评估显示，HP组的再上皮化评分高于PHT组，但在第21天，PHT组的再上皮化评分高于HP组。发现PHT组在第3天和第14天的胶原蛋白含量高于HP组。PHT组在第7天的血管化良好的肉芽组织高于其他组。与对照组相比，HP组和PHT组在所有天数的烧伤创面愈合区域中VEGF和TGF-β表达均显著增加。
局部应用HP可促进烧伤创面的再上皮化，以缩短浅度烧伤的创面愈合时间。另一方面，苯妥英通过增加血管化肉芽组织和通过再上皮化促进胶原蛋白合成来促进愈合。PHT和HP治疗后VEGF和TGF-β表达的增加强烈表明，PHT和HP治疗可促进烧伤创面区域VEGF和TGF-β的产生和作用。
