Turner D L, Snyder E Y, Cepko C L
Program in Neuroscience, Harvard Medical School, Boston, Massachusetts 02115.
Neuron. 1990 Jun;4(6):833-45. doi: 10.1016/0896-6273(90)90136-4.
We previously used a retroviral vector to mark clones in the postnatal rodent retina and showed that at least two types of neurons and Müller glia can arise from a common progenitor. Here we describe the use of exo utero surgery to introduce a marker retrovirus into the proliferative zone of the retinas of embryonic day 13 and 14 mice. Analysis of marked clones in the resulting adult retinas shows that almost all progenitor cells that continued mitosis were multipotential and that a single progenitor can generate most retinal cell types. The size of marked clones indicates that retinal cells do not employ a stem cell mode of division, but instead, both daughter cells of a progenitor can continue to divide. These results suggest that cell type determination in the rodent retina is independent of lineage. We propose a model for the generation of retinal cell types in which the cessation of mitosis and cell type determination are independent events, controlled by environmental interactions.
我们之前使用逆转录病毒载体标记出生后啮齿动物视网膜中的克隆，并表明至少两种类型的神经元和 Müller 胶质细胞可源自共同的祖细胞。在此，我们描述了使用宫外手术将标记逆转录病毒引入胚胎第 13 天和 14 天小鼠视网膜的增殖区。对所得成年视网膜中标记克隆的分析表明，几乎所有继续进行有丝分裂的祖细胞都是多能的，并且单个祖细胞可以产生大多数视网膜细胞类型。标记克隆的大小表明视网膜细胞不采用干细胞分裂模式，相反，祖细胞的两个子细胞都可以继续分裂。这些结果表明啮齿动物视网膜中的细胞类型决定与谱系无关。我们提出了一个视网膜细胞类型生成模型，其中有丝分裂的停止和细胞类型的决定是独立事件，由环境相互作用控制。
