De Meirsman C, Van Soom C, Verreth C, Van Gool A, Vanderleyden J
F.A. Janssens Memorial Laboratory of Genetics, University of Leuven, Heverlee, Belgium.
Plasmid. 1990 Nov;24(3):227-34. doi: 10.1016/0147-619x(90)90006-x.
We have determined the nucleotide sequence of IS427, an insertion sequence from Agrobacterium tumefaciens T37, IS427 is 1271 bp long, contains 16-bp imperfect terminal inverted repeats, and generates a 2-bp target sequence duplication. It is present at three sites in the pTiT37 plasmid and is absent from the chromosome of A. tumefaciens T37. Each of the IS427 elements sequenced was near a site with sequence homology to integration host factor (IHF)-binding sites which suggested that IHF may be involved in IS427 transposition.
我们已经确定了来自根癌农杆菌T37的插入序列IS427的核苷酸序列。IS427长1271 bp，包含16 bp的不完全末端反向重复序列，并产生2 bp的靶序列重复。它存在于pTiT37质粒的三个位点，而在根癌农杆菌T37的染色体中不存在。测序的每个IS427元件都靠近一个与整合宿主因子（IHF）结合位点具有序列同源性的位点，这表明IHF可能参与IS427的转座。
