铜绿假单胞菌插入序列IS222的核苷酸序列：IS3家族的另一个成员。

Nucleotide sequence of the Pseudomonas aeruginosa insertion sequence IS222: another member of the IS3 family.

作者信息

Kropinski A M, Farinha M A, Jansons I

机构信息

Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada.

出版信息

Plasmid. 1994 Mar;31(2):222-8. doi: 10.1006/plas.1994.1024.

DOI:10.1006/plas.1994.1024

PMID:8029330

Abstract

Sequence analysis of the Pseudomonas aeruginosa insertion sequence element IS222 revealed it to be 1234 bp in size with 23 bp imperfect terminal inverted repeats. Insertion caused a 5-bp duplication of the insertion site. Two ORFs were identified, one of which, ORFA, could encode a basic (pI 10.5) polypeptide with a mass of 11,709. This sequence bears strong homology to the putative ORFA product from the Shigella dysenteriae insertion sequence element IS911, which is a member of the IS3 family of insertion elements. As with other members of this group the nucleotide sequence contains a "frameshift window" (AAAAAAG; M. Chandler and O. Fayet (1993). Mol. Microbiol. 7, 497-503) at which ribosome slippage can result in a fusion protein (ORFAB).

摘要

铜绿假单胞菌插入序列元件IS222的序列分析表明，其大小为1234 bp，具有23 bp的不完全末端反向重复序列。插入导致插入位点出现5 bp的重复。鉴定出两个开放阅读框（ORF），其中一个ORFA可编码一种碱性（pI 10.5）多肽，质量为11709。该序列与痢疾志贺菌插入序列元件IS911的推定ORFA产物具有高度同源性，IS911是插入元件IS3家族的成员。与该组的其他成员一样，核苷酸序列包含一个“移码窗口”（AAAAAAG；M. Chandler和O. Fayet（1993年）。《分子微生物学》7，497 - 503），在该窗口处核糖体滑动可导致融合蛋白（ORFAB）的产生。