Department of Chemistry & Biochemistry, Gonzaga University, Spokane, Washington 99258, United States.
Langmuir. 2011 Jul 19;27(14):8634-44. doi: 10.1021/la200952c. Epub 2011 Jun 15.
A method is presented for the assembly of lipid bilayers on silica colloids via reconstitution of dried lipid films solvent-cast from chloroform within packed beds of colloids ranging from 100 nm to 10 μm in diameter. Rapid solvent evaporation from the packed bed void volume results in uniform distribution of dried lipid throughout the colloidal bed. Fluorescence measurements indicate that significant, if not quantitative, retention of DOPC or DPPC films cast between sub-bilayer and multilayer quantities occurs when the colloids are redispersed in aqueous solution. Phospholipid bilayers assembled in this manner are shown to effectively passivate the surface of 250 nm colloids to nonspecific adsorption of bovine serum albumin. The method is shown to be capable of preparing supported bilayers on colloid surfaces that do not generally support vesicle fusion such as poly(ethylene glycol) (PEG) modified silica colloids. Bilayers of lipids that have not been reported to self-assemble by vesicle fusion, including gel-phase lipids and single-chain diacetylene amphiphiles, can also be formed by this method. The utility of the solid-core support is demonstrated by the facile assembly of supported lipid bilayers within fused silica capillaries to generate materials that are potentially suitable for the analysis of membrane interactions in a microchannel format.
提出了一种在二氧化硅胶体上组装脂质双层的方法，方法是通过从氯仿中溶剂浇铸干燥的脂质膜，再将其在直径为 100nm 至 10μm 的胶体填充床中进行组装。从填充床空隙体积中快速蒸发溶剂会导致干燥的脂质在整个胶体床中均匀分布。荧光测量表明，当胶体重新分散在水溶液中时，如果不是定量的，则保留了亚单层和多层之间的 DOPC 或 DPPC 膜的相当大的部分。以这种方式组装的磷脂双层被证明可以有效地钝化 250nm 胶体的表面，防止牛血清白蛋白的非特异性吸附。该方法被证明能够在通常不支持囊泡融合的胶体表面上制备支撑双层，例如聚（乙二醇）（PEG）修饰的二氧化硅胶体。通过这种方法也可以形成那些通过囊泡融合未被报道能自组装的脂质双层，包括凝胶相脂质和单链二乙炔两亲物。固体核支撑的实用性通过在熔融二氧化硅毛细管内组装支撑脂质双层来证明，这生成了潜在适用于在微通道格式下分析膜相互作用的材料。
