Department of Biomolecular Engineering, University of California Santa Cruz, 1156 High Street, Santa Cruz, CA 95064, USA.
Biosens Bioelectron. 2011 Jul 15;26(11):4503-7. doi: 10.1016/j.bios.2011.05.010. Epub 2011 May 12.
Signal Transduction by Ion NanoGating (STING) is a label-free technology based on functionalized quartz nanopipettes. The nanopipette pore can be decorated with a variety of recognition elements and the molecular interaction is transduced via a simple electrochemical system. A STING sensor can be easily and reproducibly fabricated and tailored at the bench starting from inexpensive quartz capillaries. The analytical application of this new biosensing platform, however, was limited due to the difficult correlation between the measured ionic current and the analyte concentration in solution. Here we show that STING sensors functionalized with aptamers allow the quantitative detection of thrombin. The binding of thrombin generates a signal that can be directly correlated to its concentration in the bulk solution.
基于功能化石英纳流管的离子纳米门控（STING）信号转导是一种无标记技术。纳流管孔可以用各种识别元件进行修饰，分子相互作用通过简单的电化学系统进行转导。STING 传感器可以很容易地从廉价的石英毛细管开始在实验台上进行重复制作和定制。然而，由于在溶液中测量的离子电流与分析物浓度之间难以相关，这种新的生物传感平台的分析应用受到限制。在这里，我们展示了用适体功能化的 STING 传感器可以定量检测凝血酶。凝血酶的结合产生一个可以直接与其在溶液中的浓度相关的信号。
