Jones S S, D'Andrea A D, Haines L L, Wong G G
Genetics Institute, Cambridge, MA 02140.
Blood. 1990 Jul 1;76(1):31-5.
We have isolated the human homologue of the murine erythropoietin receptor (mEPO-R) from an erythroleukemia line, OCIM1, and from fetal liver. Both the cDNA and protein sequence of the human receptor were 82% homologous to the mEPO-R. Heterologous expression of the human cDNA in COS cells yielded a protein of about 66 Kd. The protein could be specifically immunoprecipitated with either an antibody raised against the amino terminus of mEPO-R or by a monoclonal antibody that bound EPO bound to its receptor. Cross-linking of radioiodinated EPO to COS cells expressing the human EPO-R gave apparent molecular weights of 66 and 100 Kd for the receptor. The murine interleukin-3-dependent pre-B-lymphocyte cell line, Ba/F3, was made EPO-dependent by transfection of the human cDNA into the cells and selecting for growth in EPO-containing media.
我们已从红白血病细胞系OCIM1和胎儿肝脏中分离出小鼠促红细胞生成素受体（mEPO-R）的人类同源物。人类受体的cDNA和蛋白质序列与mEPO-R的同源性均为82%。人类cDNA在COS细胞中的异源表达产生了一种约66 Kd的蛋白质。该蛋白质可用针对mEPO-R氨基末端产生的抗体或与结合了EPO的受体结合的单克隆抗体进行特异性免疫沉淀。将放射性碘化的EPO与表达人类EPO-R的COS细胞进行交联，得到受体的表观分子量为66 Kd和100 Kd。通过将人类cDNA转染到小鼠白细胞介素-3依赖的前B淋巴细胞系Ba/F3细胞中，并在含EPO的培养基中筛选生长，使该细胞系对EPO产生依赖。
