Gui K
Zhonghua Yi Xue Za Zhi. 1990 Mar;70(3):140-2, 12.
The p 21 product of ras oncogene has been detected immunohistochemically in normal, inflammatory, benign and malignant human thyroid tissues. With the monoclonal antibody SCI-oncogene I and an avidin-biotin-peroxidase complex (ABC), the expression of ras p 21 was evaluated in paraffin-embedded sections. The results showed that papillary and follicular adenocarcinomas of the thyroid had moderate to intense staining for ras p 21 in most cases. Cytoplasmic and apical surface staining were the most common patterns of immunoreactivity. Adenomas showed slight positive or negative staining in cytoplasm. Normal thyroid tissues and thyroiditis were uniformly negative. Grave's disease revealed slight to moderate staining in some cases. These findings suggest that ras oncogene is involved in carcinogenesis of thyroid carcinomas. Enhanced expression of ras p 21 may be useful in differentiation of thyroid adenocarcinomas from adenomas and may be a valuable parameter in evaluating biological behavior of tumors.
已通过免疫组织化学方法在正常、炎症、良性及恶性人甲状腺组织中检测到ras癌基因的p21产物。使用单克隆抗体SCI-癌基因I和抗生物素蛋白-生物素-过氧化物酶复合物（ABC），在石蜡包埋切片中评估ras p21的表达。结果显示，大多数情况下，甲状腺乳头状癌和滤泡状腺癌对ras p21呈中度至强染色。细胞质和顶端表面染色是最常见的免疫反应模式。腺瘤在细胞质中显示轻微阳性或阴性染色。正常甲状腺组织和甲状腺炎均为阴性。在某些病例中，格雷夫斯病显示轻微至中度染色。这些发现表明ras癌基因参与甲状腺癌的致癌过程。ras p21表达增强可能有助于甲状腺腺癌与腺瘤的鉴别，并且可能是评估肿瘤生物学行为的一个有价值的参数。
