Biotechnology and Bioindustry Center, Colombian Corporation for Agricultural Research, CORPOICA, Mosquera, Cundinamarca Colombia.
Genet Mol Biol. 2010 Apr;33(2):271-8. doi: 10.1590/S1415-47572010005000016. Epub 2010 Jun 1.
The Lulo or naranjilla (Solanum quitoense Lam.) and the tree tomato or tamarillo (Solanum betaceum Cav. Sendt.) are both Andean tropical fruit species with high nutritional value and the potential for becoming premium products in local and export markets. Herein, we present a report on the genetic characterization of 62 accessions of lulos (n = 32) and tree tomatoes (n = 30) through the use of PCR-based markers developed from single-copy conserved orthologous genes (COSII) in other Solanaceae (Asterid) species. We successfully PCR amplified a set of these markers for lulos (34 out of 46 initially tested) and tree tomatoes (26 out of 41) for molecular studies. Six polymorphic COSII markers were found in lulo with a total of 47 alleles and five polymorphic markers in tree tomato with a total of 39 alleles in the two populations. Further genetic analyses indicated a high population structure (with F(ST) > 0.90), which may be a result of low migration between populations, adaptation to various niches and the number of markers evaluated. We propose COSII markers as sound tools for molecular studies, conservation and the breeding of these two fruit species.
莱洛果（Solanum quitoense Lam.）和树番茄（Solanum betaceum Cav. Sendt.）均为安第斯热带水果，具有较高的营养价值，有潜力成为本地和出口市场的优质产品。本文报告了对 62 份莱洛果（n = 32）和树番茄（n = 30）样本的遗传特征进行的研究，这些样本是通过使用在其他茄科（合瓣花）物种中发现的单拷贝保守直系同源基因（COSII）开发的基于 PCR 的标记进行分析的。我们成功地对莱洛果（最初测试的 46 个中有 34 个）和树番茄（最初测试的 41 个中有 26 个）的一组这些标记进行了 PCR 扩增，用于分子研究。在这两个群体中，在莱洛果中发现了 6 个多态性的 COSII 标记，共有 47 个等位基因，在树番茄中发现了 5 个多态性标记，共有 39 个等位基因。进一步的遗传分析表明，种群结构高度分化（F(ST) > 0.90），这可能是由于种群间迁移率低、对各种小生境的适应以及评估的标记数量较少所致。我们提出，COSII 标记是对这两种水果进行分子研究、保护和育种的可靠工具。
