Institute of Maize Research, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
Plant Cell Rep. 2013 Mar;32(3):379-88. doi: 10.1007/s00299-012-1371-4. Epub 2012 Dec 12.
We develop a set of universal genetic markers based on single-copy orthologous (COSII) genes in Poaceae. Being evolutionary conserved, single-copy orthologous (COSII) genes are particularly useful in comparative mapping and phylogenetic investigation among species. In this study, we identified 2,684 COSII genes based on five sequenced Poaceae genomes including rice, maize, sorghum, foxtail millet, and brachypodium, and then developed 1,072 COSII markers whose transferability and polymorphism among five bamboo species were further evaluated with 46 pairs of randomly selected primers. 91.3 % of the 46 primers obtained clear amplification in at least one bamboo species, and 65.2 % of them produced polymorphism in more than one species. We also used 42 of them to construct the phylogeny for the five bamboo species, and it might reflect more precise evolutionary relationship than the one based on the vegetative morphology. The results indicated a promising prospect of applying these markers to the investigation of genetic diversity and the classification of Poaceae. To ease and facilitate access of the information of common interest to readers, a web-based database of the COSII markers is provided ( http://www.sicau.edu.cn/web/yms/PCOSWeb/PCOS.html ).
我们基于禾本科植物的单拷贝直系同源（COSII）基因开发了一套通用的遗传标记。单拷贝直系同源（COSII）基因在物种间的比较作图和系统发育研究中具有进化保守性，因此特别有用。在这项研究中，我们基于包括水稻、玉米、高粱、谷子和柳枝稷在内的 5 种禾本科植物测序基因组，鉴定了 2684 个 COSII 基因，然后开发了 1072 个 COSII 标记，并用 46 对随机选择的引物进一步评估了它们在 5 种竹子中的转移能力和多态性。在至少一种竹子中，46 对引物中的 91.3%获得了清晰的扩增，其中 65.2%在超过一种物种中产生了多态性。我们还使用其中的 42 对引物构建了这 5 种竹子的系统发育树，它可能比基于营养形态的系统发育树更能反映出精确的进化关系。这些结果表明，这些标记在禾本科植物的遗传多样性研究和分类中有很好的应用前景。为了方便读者获取共同感兴趣的信息，我们提供了一个基于网络的 COSII 标记数据库（http://www.sicau.edu.cn/web/yms/PCOSWeb/PCOS.html）。
