Laboratorio de Herpetología, Departamento de Biología, Facultad de Ciencias Exactas y Naturales y Agrimensura, Universidad Nacional del Nordeste, Corrientes, Argentina.
Genet Mol Biol. 2011 Jan;34(1):84-7. doi: 10.1590/S1415-47572010005000116. Epub 2011 Mar 1.
In this work we analyzed the karyotype of five populations of Adenomera diptyx from Argentina after conventional staining, Ag-NOR and C-banding. All specimens presented 2n = 26 and FN = 34. The karyotype was formed by three submetacentric, one metacentric and nine telocentric pairs. Silver staining revealed that the NOR was located on a secondary constriction in pair 7. C- banding evidenced constitutive heterochromatin at the pericentromeric region of all chromosomes. The karyotype of A. diptyx was similar to that of A. hylaedactyla (2n = 26, FN = 34) and different from that of A. andreae (2n = 26, FN = 40) in the fundamental number and secondary constriction position. It also differed from the karyotypes of A. marmorata (2n = 24, FN = 34 and 36) and of A. aff. bokermanni (2n = 23, FN = 34) in diploid number. Until a comprehensive cytogenetic analysis of all the species of the genus is performed, their chromosome evolution will remain poorly understood.
在这项工作中,我们对来自阿根廷的五个 Adenomera diptyx 种群的染色体组型进行了分析,方法是常规染色、Ag-NOR 和 C-带。所有标本的 2n = 26,FN = 34。核型由三个亚中部着丝粒、一个中部着丝粒和九个端着丝粒对组成。银染显示 NOR 位于第 7 对的次缢痕上。C-带带型显示所有染色体的着丝粒周围区域都有组成性异染色质。A. diptyx 的核型与 A. hylaedactyla(2n = 26,FN = 34)相似,而与 A. andreae(2n = 26,FN = 40)在基数和次缢痕位置上不同。它还与 A. marmorata(2n = 24,FN = 34 和 36)和 A. aff. bokermanni(2n = 23,FN = 34)的核型在二倍体数量上不同。在对该属的所有物种进行全面的细胞遗传学分析之前,它们的染色体进化仍知之甚少。