Silva A P, Haddad C F, Kasahara S
Departamento de Biologia, Instituto de Biociências, Universidade Estadual Paulista, Rio Claro, São Paulo, Brazil.
Cytobios. 2000;103(402):25-38.
Cytogenetic studies were carried out on five species of Leptodactylus, namely L. fuscus, L. notoaktites, L. labyrinthicus, L. ocellatus, and L. podicipinus, after standard staining, Ag-NOR and C-banding as well as BrdU incorporation for three of them. The species had 2n = 22 chromosomes and two basic karyotype patterns. Chromosome 8 was a marker bearing a secondary constriction. In all species, this secondary constriction corresponded to the Ag-NOR site. The species had centromeric C-bands in all chromosomes of the complement, but some interstitial or telomeric bands seemed to differentiate some karyotypes, either at the species or the population level. In L. ocellatus, the C-banding pattern confirmed the occurrence of a heteromorphic pericentric inversion in chromosome 8 in specimens from one of the populations. The BrdU incorporation technique showed no detectable difference in the replication patterns of the major bands in the chromosomes of L. notoaktites, L. labyrinthicus, and L. ocellatus.
对五种细趾蟾属物种进行了细胞遗传学研究，这五个物种分别是深色细趾蟾、诺氏细趾蟾、迷宫细趾蟾、眼斑细趾蟾和蹼足细趾蟾。在进行标准染色、银染核仁组织区（Ag-NOR）和C带分析后，对其中三个物种还进行了5-溴脱氧尿嘧啶核苷（BrdU）掺入实验。这些物种的染色体数目均为2n = 22，有两种基本核型模式。第8号染色体是带有次缢痕的标记染色体。在所有物种中，该次缢痕均对应于Ag-NOR位点。这些物种的染色体组中所有染色体均有着丝粒C带，但一些居间或端粒带似乎在物种或种群水平上使某些核型有所区别。在眼斑细趾蟾中，C带模式证实了来自其中一个种群的标本中第8号染色体存在异态的臂间倒位。BrdU掺入技术显示，诺氏细趾蟾、迷宫细趾蟾和眼斑细趾蟾染色体中主要带的复制模式没有可检测到的差异。
