Laboratório de Biodiversidade Molecular e Conservação, Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP, Brazil.
Genet Mol Biol. 2011 Jan;34(1):173-5. doi: 10.1590/S1415-47572010005000111. Epub 2011 Mar 1.
Leontopithecus caissara is a critically endangered primate species from the Brazilian Atlantic Forest. Nineteen microsatellite loci, previously developed for congeneric species, were tested with 34 L. caissara individuals from Superagüi Island. Of the 19 loci, 17 (89.4%) produced robust alleles, nine (47.4%) of these proved to be polymorphic, with a total of 23 alleles and an average of 2.56 alleles per locus. Expected and observed heterozygosity averaged 0.483 and 0.561, respectively. The exclusion power for identifying the first parent of an arbitrary offspring was 0.315 over all loci. The results thus indicate both the usefulness and limitations of these nine microsatellite loci in the genetic analysis of L. caissara, as well as their potentiality for genetic investigation in other congeneric species.
栗喉蜂虎是一种极度濒危的灵长类物种,来自巴西大西洋森林。先前为同属物种开发的 19 个微卫星基因座,经 34 只来自 Superagüi 岛的栗喉蜂虎个体检测。在这 19 个基因座中,有 17 个(89.4%)产生了稳定的等位基因,其中 9 个(47.4%)具有多态性,共产生了 23 个等位基因,每个基因座的平均等位基因数为 2.56。预期和观察到的杂合度分别平均为 0.483 和 0.561。所有基因座的第一亲排除率为 0.315。因此,这些九个微卫星基因座在栗喉蜂虎的遗传分析中既具有实用性和局限性,也具有在其他同属物种中进行遗传研究的潜力。