Department of Molecular Biology, Faculty of Science and Arts, Namik Kemal University, Tekirdag Turkey.
Genet Mol Biol. 2010 Jan;33(1):1-4. doi: 10.1590/S1415-47572009005000102. Epub 2010 Mar 1.
Recently, efforts have been focused on mitochondrial DNA changes and their relation to human cancers. Among them, a 4977 bp deletion of mitochondrial DNA, named "common deletion", has been investigated in several types of tumors, with inconsistent results. In this study, we investigated the presence of the common deletion in tissues from 25 breast, 25 colorectal and 50 thyroid tumors and in the adjacent healthy tissues from Turkish patients. Samples from healthy volunteers were also evaluated for comparison. Two PCR-based methods were used for the detection of the common deletion. First, two pairs of primers were used to amplify wild-type and deleted mtDNA. Then, a highly sensitive nested-PCR was performed, to determine low amounts of deleted genomes. By the first method, wild-type mtDNAs were observed in all samples, but a deletion was observed in only six thyroid samples, by using the nested-PCR method. In conclusion, the mitochondrial common deletion was very rare in our study group and did not appear to be not related with cancer.
最近，人们致力于研究线粒体 DNA 变化及其与人类癌症的关系。其中，一种名为“常见缺失”的线粒体 DNA 4977bp 缺失已在多种类型的肿瘤中进行了研究，但结果不一致。在本研究中，我们检测了 25 例乳腺癌、25 例结直肠癌和 50 例甲状腺肿瘤组织及其土耳其患者邻近健康组织中常见缺失的存在情况。同时还评估了健康志愿者的样本作为对照。我们使用了两种基于 PCR 的方法来检测常见缺失。首先，使用两对引物扩增野生型和缺失型 mtDNA。然后，进行高度敏感的巢式 PCR，以确定低量的缺失基因组。通过第一种方法，在所有样本中都观察到了野生型 mtDNA，但通过巢式 PCR 方法仅在 6 例甲状腺样本中观察到了缺失。总之，在我们的研究组中，线粒体常见缺失非常罕见，似乎与癌症无关。
