Division of Plant Industry, CSIRO, Canberra, Australian Capital Territory, Australia.
PLoS One. 2011;6(5):e20408. doi: 10.1371/journal.pone.0020408. Epub 2011 May 26.
Mutants in the rice PLASTOCHRON 3 and maize VIVIPAROUS 8 genes have been shown to have reduced dormancy and ABA levels. In this study we used several mutants in the orthologous gene ALTERED MERISTEM PROGRAM 1 (AMP1) to determine its role in seed dormancy in Arabidopsis. Here we report that there are accession-specific effects of mutations in AMP1. In one accession, amp1 mutants produce seeds with higher dormancy, while those in two other accessions produce seeds of lower dormancy. These accession-specific effects of mutating AMP1 were shown to extend to ABA levels. We assayed global gene transcription differences in seeds of wild-type and mutant from two accessions demonstrating opposing phenotypes. The transcript changes observed indicate that the amp1 mutation shifts the seed transcriptome from a dormant into an after-ripened state. Specific changes in gene expression in the mutants give insight into the direct and indirect effects that may be contributing to the opposing dormancy phenotypes observed, and reveal a role for AMP1 in the acquisition and/or maintenance of seed dormancy in Arabidopsis.
已证实，水稻 PLASTOCHRON 3 和玉米 VIVIPAROUS 8 基因的突变体休眠期和 ABA 水平降低。在这项研究中，我们使用了几个与拟南芥中同源基因 ALTERED MERISTEM PROGRAM 1（AMP1）的突变体，以确定其在种子休眠中的作用。我们报告称，AMP1 突变存在特定于访问者的影响。在一个访问者中，amp1 突变体产生的种子休眠期更高，而在另外两个访问者中产生的种子休眠期更低。AMP1 突变的这些特定于访问者的影响延伸到 ABA 水平。我们检测了来自两个具有相反表型的访问者的野生型和突变体种子的全局基因转录差异。观察到的转录变化表明，amp1 突变将种子转录组从休眠状态转变为后熟状态。突变体中基因表达的特定变化深入了解了可能导致观察到的休眠表型相反的直接和间接影响，并揭示了 AMP1 在拟南芥中获取和/或维持种子休眠中的作用。
