Rose-John S, Schooltink H, Lenz D, Hipp E, Dufhues G, Schmitz H, Schiel X, Hirano T, Kishimoto T, Heinrich P C
Institut für Biochemie der Rheinisch-Westfälischen Technischen Hochschule Aachen, Federal Republic of Germany.
Eur J Biochem. 1990 May 31;190(1):79-83. doi: 10.1111/j.1432-1033.1990.tb15548.x.
Affinity cross-linking of 125I-labeled recombinant human interleukin-6 (IL-6) to human hepatoma cells (HepG2) allowed the detection of three IL-6-containing complexes with molecular masses of 100 kDa, 120 kDa and 200 kDa. Treatment of HepG2 cells with dexamethasone led to a time- and dose-dependent up-regulation of IL-6-receptor mRNA levels. By the use of cross-linking this effect was also seen at the protein level, where all three IL-6-binding complexes increased upon incubation of HepG2 cells with dexamethasone. Under conditions of IL-6-receptor up-regulation by dexamethasone, gamma-fibrinogen mRNA induction by IL-6 is stronger and occurs earlier than without dexamethasone. We propose therefore that the expression of the IL-6 receptor might be a rate-limiting step in acute-phase-protein induction.
将125I标记的重组人白细胞介素-6（IL-6）与人类肝癌细胞（HepG2）进行亲和交联，可检测到三种分子量分别为100 kDa、120 kDa和200 kDa的含IL-6复合物。用地塞米松处理HepG2细胞会导致IL-6受体mRNA水平呈时间和剂量依赖性上调。通过交联技术，在蛋白质水平也观察到了这种效应，即用HepG2细胞与地塞米松孵育后，所有三种IL-6结合复合物均增加。在地塞米松上调IL-6受体的条件下，IL-6诱导γ-纤维蛋白原mRNA的作用比未用地塞米松时更强且更早出现。因此，我们认为IL-6受体的表达可能是急性期蛋白诱导过程中的一个限速步骤。
