Bresler S E, Bresler V M, Kazbekov E N, Nikiforov A A, Vasilieva N N
Biochim Biophys Acta. 1979 Jan 5;550(1):110-9. doi: 10.1016/0005-2736(79)90119-6.
The kinetics of active transport of an organic acid (fluorescein) through the membranes of the choroid plexus from the lateral ventricules of the brain of rabbit was studied both morphologically and functionally. It was shown that fluorescein is actively translocated through the apical and basal membrane of the epithelium and is accumulated in blood capillaries at a concentration exceeding one order of magnitude that in the incubation medium. The kinetic curves displaying saturation and the demonstration of inhibition by other acids shows that a specific carrier is involved in the transfer across the membrane. The active transport of fluorescein at 20 degrees C was found to be sodium independent. Total exclusion of sodium from the incubation medium does not change the Michaelis constant (Km) and maximal velocity (V). The active transport depends on the operation of (Na+ + K+)-ATPase as energy source but obviously no specific complexes with the participation of sodium are involved.
对有机酸（荧光素）从兔脑侧脑室经脉络丛膜进行主动转运的动力学进行了形态学和功能学研究。结果表明，荧光素通过上皮细胞的顶端膜和基底膜进行主动转运，并在血毛细血管中积累，其浓度超过孵育介质中的浓度一个数量级以上。显示饱和的动力学曲线以及其他酸的抑制作用表明，跨膜转运涉及一种特异性载体。发现在20℃时荧光素的主动转运不依赖于钠。从孵育介质中完全去除钠不会改变米氏常数（Km）和最大速度（V）。主动转运依赖于（Na+ + K+）-ATP酶作为能量来源的运作，但显然不涉及钠参与的特异性复合物。
