Otte A P, Roy D, Siemerink M, Koster C H, Hochstenbach F, Timmermans A, Durston A J
Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht.
J Cell Biol. 1990 Jul;111(1):271-8. doi: 10.1083/jcb.111.1.271.
We characterized a novel extracellular matrix element that is present in the earliest developmental stages of Xenopus laevis, and is recognized by an mAb 3D7. Based on amino acid composition, breakdown patterns by bacterial collagenases, and the molecular weights of the components of the antigen (240, 200, and 140 kD), we found it very similar to mammalian collagen type VI. The antigen is evenly distributed in unfertilized eggs. Shortly after fertilization, it becomes localized intracellularly in the periphery of the cleaving embryo as well as in the extracellular spaces. During gastrulation, the antigen was localized in the cells lining the blastopore and in the extracellular space between the two cell layers, in the presumptive archenteron. When Fab elements of the 3D7 antibody were added to the culture medium, gastrulation was blocked, suggesting a role for the antigen in gastrulation movements.
我们鉴定了一种新的细胞外基质成分，它存在于非洲爪蟾发育的最早阶段，并可被单克隆抗体3D7识别。根据氨基酸组成、细菌胶原酶的降解模式以及抗原成分的分子量（240、200和140 kD），我们发现它与哺乳动物的VI型胶原非常相似。该抗原在未受精卵中均匀分布。受精后不久，它在分裂胚胎的外周细胞内以及细胞外空间中定位。在原肠胚形成期间，该抗原定位于胚孔边缘的细胞以及两个细胞层之间的细胞外空间中，即预定的原肠。当将3D7抗体的Fab片段添加到培养基中时，原肠胚形成被阻断，这表明该抗原在原肠胚形成运动中起作用。
