Matthew W D, Reichardt L F
J Immunol Methods. 1982;50(3):239-53. doi: 10.1016/0022-1759(82)90162-4.
A simple method is described for generating active molecules with molecular weights between 110 and 230 kilodaltons from mouse immunoglobulin M. The majority of these molecules have a 1 : 1 ratio of intact heavy and light chains. Approximately 70% of the specific IgM protein will still bind antigen after digestion with only a small decrease in binding affinity. Both anti-kappa and anti-mu chain specific antisera recognize these molecules. These low molecular weight molecules are much more efficient in immunocytochemistry and have localized antigens that could not be detected with undigested IgM.
本文描述了一种简单方法，可从小鼠免疫球蛋白M生成分子量在110至230千道尔顿之间的活性分子。这些分子中的大多数重链和轻链完整，比例为1:1。仅经过消化后，约70%的特异性IgM蛋白仍能结合抗原，且结合亲和力仅有小幅下降。抗κ链和抗μ链特异性抗血清均可识别这些分子。这些低分子量分子在免疫细胞化学中效率更高，并且能够定位未消化的IgM无法检测到的抗原。
