Maize Research Institute, Sichuan Agricultural University, Chengdu, China.
Carbohydr Res. 2011 Sep 27;346(13):1684-91. doi: 10.1016/j.carres.2011.05.003. Epub 2011 May 10.
Starch is the major carbon reserve in plant storage organs, the synthesis of which is orchestrated by four major enzymes, ADP-glucose pyrophosphorylase, starch synthase, starch-branching enzyme and starch-debranching enzyme. There is much information available on the function of these key enzymes; however, little is known about their transcriptional regulation. In order to understand the transcriptional regulation of starch biosynthesis, the expression profiles of 24 starch genes were investigated in this work. The results showed major transcriptional changes for 15 of the 24 starch genes observed in maize endosperm, most of which are elevated at the early and middle stages of the developing endosperm. Sucrose, abscisic acid (ABA) and indole-3-acetic acid (IAA) had a significant correlation with the expression of 15 genes, indicating that sugars and phytohormones might take part in the regulation of starch synthesis. Also, we found that there is interaction of abscisic acid and sucrose on the regulation of the expression of these genes.
淀粉是植物贮藏器官中主要的碳储备，其合成由四种主要酶调控：ADP-葡萄糖焦磷酸化酶、淀粉合酶、淀粉分支酶和淀粉去分支酶。这些关键酶的功能已有很多信息，但它们的转录调控知之甚少。为了了解淀粉生物合成的转录调控，本研究调查了 24 个淀粉基因的表达谱。结果表明，在玉米胚乳中观察到的 24 个淀粉基因中的 15 个主要发生转录变化，其中大多数在胚乳发育的早期和中期升高。蔗糖、脱落酸（ABA）和吲哚-3-乙酸（IAA）与 15 个基因的表达呈显著相关，表明糖和植物激素可能参与淀粉合成的调节。此外，我们发现 ABA 和蔗糖对这些基因表达的调节存在相互作用。
