Ren X D, Liu H M, Liu Y H, Hu Y F, Zhang J J, Huang Y B
College of Life Science, Sichuan Agricultural University, Ya'an, China.
Maize Research Institute, Sichuan Agricultural University, Chengdu, China.
Genet Mol Res. 2015 Mar 6;14(1):1671-8. doi: 10.4238/2015.March.6.13.
Starch is the major storage product in the endosperm of cereals. Its synthesis is closely related to sucrose metabolism. In our previous study, we found that the expression of most of the genes involved in starch synthesis might be regulated by sugars and hormones in the maize endosperm. However, little is known regarding the transcriptional regulation of genes involved in sucrose metabolism. Thus, in this study, maize endosperms were treated with different sugars and hormones and the expression of genes involved in sucrose metabolism (including synthesis, degradation, and transport) were evaluated using real-time quantitative reverse transcription-polymerase chain reaction. We found that genes affected by different sugars and hormones were primarily regulated by abscisic acid. Sucrose and abscisic acid showed an additive effect on the expression of some genes. Differences in the transcriptional regulation of genes involved in sucrose metabolism and starch biosynthesis were observed.
淀粉是谷物胚乳中的主要储存产物。其合成与蔗糖代谢密切相关。在我们之前的研究中，我们发现玉米胚乳中大多数参与淀粉合成的基因的表达可能受糖类和激素调控。然而，关于参与蔗糖代谢的基因的转录调控知之甚少。因此，在本研究中，用不同的糖类和激素处理玉米胚乳，并使用实时定量逆转录 - 聚合酶链反应评估参与蔗糖代谢（包括合成、降解和转运）的基因的表达。我们发现受不同糖类和激素影响的基因主要受脱落酸调控。蔗糖和脱落酸对一些基因的表达表现出累加效应。观察到参与蔗糖代谢和淀粉生物合成的基因在转录调控上存在差异。
