Departamento de Anatomia, Biologia Celular e Fisiologia e Biofísica, Instituto de Biologia, Universidade Estadual de Campinas, CEP, 13083-863 Campinas, SP, Brazil.
Micron. 2011 Dec;42(8):801-7. doi: 10.1016/j.micron.2011.04.006. Epub 2011 May 18.
Wings of the butterflies Morpho aega and Eryphanis reevesi were investigated in the present study by fluorescence, polarization and infra-red (IR) spectroscopic microscopy with the aim of identifying the oriented organization of their components and morphological details of their substructures. These wings were found to exhibit a strong iridescent glow depending on the angle of the incident light; their isolated scales exhibited blue fluorescence. Parallel columns or ridges extend from the pad and sockets to the dented apical scale's region, and they are perpendicular to the ribs that connect the columnar ridges. The scales reveal linear dichroism (LD) visually, when attached on the wing matrix or isolated on slides. The LD was inferred to be textural and positive and was also demonstrated with IR microscopy. The scale columns and ribs are birefringent structures. Images obtained before and after birefringence compensation allowed a detailed study of the scale morphology. Form and intrinsic birefringence findings here estimated and discussed in the context of nonlinear optical properties, bring to the level of morphology the state of molecular order and periodicity of the wing structure. FT-IR absorption peaks were found at wavenumbers which correspond to symmetric and asymmetric (-N-H) stretching, symmetric (-C-H) stretching, amide I (-CO) stretching, amide II(-N-H), and β-linking. Based on LD results obtained with polarized IR the molecular vibrations of the wing scales of M. aega and E. reevesi are assumed to be oriented with respect to the long axis of these structures.
本文采用荧光、偏光和红外（IR）光谱显微镜研究了 Morpho aega 和 Eryphanis reevesi 蝴蝶的翅膀，旨在确定其成分的取向组织和亚结构的形态细节。这些翅膀在入射光角度的影响下呈现出强烈的虹彩光泽；它们的分离鳞片表现出蓝色荧光。从翅垫和窝点延伸到凹入的顶端鳞片区域的平行柱或脊，与连接柱状脊的肋骨垂直。当鳞片附着在翅膀基质上或在载玻片上分离时，从视觉上可以看出线性二色性（LD）。LD 被推断为纹理和阳性，并通过 IR 显微镜得到证明。鳞片柱和肋骨是双折射结构。在进行双折射补偿前后获得的图像允许对鳞片形态进行详细研究。在此处估计并在非线性光学性质的背景下讨论的形态和固有双折射发现，将分子有序状态和翅膀结构的周期性提升到形态水平。在波数处发现了 FT-IR 吸收峰，这些波数对应于对称和不对称（-N-H）伸缩、对称（-C-H）伸缩、酰胺 I（-CO）伸缩、酰胺 II（-N-H）和β-连接。根据用偏振 IR 获得的 LD 结果，假设 M. aega 和 E. reevesi 蝴蝶翅膀鳞片的分子振动相对于这些结构的长轴取向。
