Department of Oral and Maxillofacial Surgery, Guanghua School of Stomatology, Sun Yat-Sen University, 56 Lingyuanxi Road, Guangzhou 510055, PR China.
Oral Oncol. 2011 Jul;47(7):566-70. doi: 10.1016/j.oraloncology.2011.04.017.
The forkhead transcription factor, Foxp3, has been identified as a key player in CD4(+)CD25(+)Foxp3(+) regulatory T cells (Tregs) function and a definitive marker of Tregs. Recently, it was reported that Foxp3 could be expressed by tumor cells themselves. The present study was to investigate the expression of Foxp3 in tongue squamous cells carcinoma (TSCC) cells and its clinical significance. In this study, the expression of Foxp3 by TSCC cells was demonstrated in TSCC tissue samples and three TSCC cell lines using immunohistochemical staining, realtime-PCR and Western blotting, and its clinical significance were statistically analyzed. The immunohistochemical assay in TSCC paraffin-embedded samples showed positive staining in 48 of 81 (59.3%) cases. The expression was significantly associated with pathologic differentiation (P=0.040) and T stage (P=0.000), and furthermore, inversely associate with patient survival (P=0.021). Multivariate analysis (Cox regression) suggested that Foxp3 expression in TSCC cells was an independent prognostic indicator for TSCC (P=0.032).
叉头框转录因子 Foxp3 已被鉴定为 CD4(+)CD25(+)Foxp3(+)调节性 T 细胞 (Tregs) 功能的关键因子和 Tregs 的明确标志物。最近有报道称 Foxp3 可由肿瘤细胞自身表达。本研究旨在探讨 Foxp3 在舌鳞状细胞癌 (TSCC) 细胞中的表达及其临床意义。在这项研究中，通过免疫组织化学染色、实时 PCR 和 Western blot 分析，在 81 例 TSCC 组织样本和 3 种 TSCC 细胞系中证实了 Foxp3 在 TSCC 细胞中的表达，并对其临床意义进行了统计学分析。在 TSCC 石蜡包埋样本中的免疫组化检测显示，48 例 (59.3%) 呈阳性染色。表达与病理分级 (P=0.040) 和 T 分期 (P=0.000) 显著相关，且与患者生存呈负相关 (P=0.021)。多因素分析 (Cox 回归) 表明 Foxp3 在 TSCC 细胞中的表达是 TSCC 的独立预后指标 (P=0.032)。
