Jiang X D, Luo G, Wang X H, Chen L L, Ke X, Li Y
Department of Oral and Maxillofacial Surgery, The Affiliated Stomatological Hospital of Nanchang University, Nanchang 330006, China (Pressent address: Department of Periodontology, Stomatology Hospital of Guangzhou Medical University & Guangzhou Institute of Oral Disease & Key Laboratory of Oral Medicine, Guangzhou 510140, China).
Department of Periodontology, Stomatology Hospital of Guangzhou Medical University & Guangzhou Institute of Oral Disease & Key Laboratory of Oral Medicine, Guangzhou 510140, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2017 Jan 9;52(1):27-33. doi: 10.3760/cma.j.issn.1002-0098.2017.01.006.
To investigate Oct4 and Sox2 protein expression in tongue squamous cell carcinoma (TSCC) and the relationships between the expressions of Oct4 and Sox2 and clinical pathological characteristics and survival of patients. The paraffin imbedded tissue specimens of 51 patients with histologically confirmed TSCC were included. Immunohistochemistry was employed to detect the protein expression of Oct4 and Sox2 in 51 TSCC tissue samples. The protein expression levels of Oct4 and Sox2 and their relationships with both clinicopathological features and survival of patients with TSCC were evaluated. In 51 TSCC cases,positive expressions of Oct4 and Sox2 were mainly located in the nucleus of tumor cells. The expression of Oct4 was strongly positive in 27 cases (53%), weakly positive in 16 (31%) and negative in 8 (16%), whereas that of Sox2 was strongly positive in 25 cases (49%), weakly positive in 22 (43%) and negative in 4 (8%). Oct4 and Sox2 expression levels were significantly correlated with the histological grade of TSCC (0.004, 0.006, respectively), not correlated with age, gender, T stage, tobacco smoking and alcohol consumption status (0.05), but Oct4 expression level was significantly associated with lymph node metastasis (0.001). Sox2 expression level was not associated with lymph node metastasis (0.05). The expression of Sox2 was significantly correlated with Oct4 (0.001). Oct4 and Sox2 expression was associated with poor overall survival of patients with TSCC (0.001, 0.002, respectively), cases with higher Oct4 and Sox2 expression had the poorest overall survival (0.001). Sox2 expression and lymph node metastasis were independent prognostic factors of overall survival in patients with TSCC (0.02, 0.001, respectively). Sox2 had independent prognostic effects on overall survival, suggesting that Sox2 expression may be an usefull indicator for predicting the prognosis of patients with TSCC.
研究Oct4和Sox2蛋白在舌鳞状细胞癌（TSCC）中的表达情况，以及Oct4和Sox2表达与患者临床病理特征及生存情况之间的关系。纳入51例经组织学确诊为TSCC的石蜡包埋组织标本。采用免疫组织化学方法检测51例TSCC组织样本中Oct4和Sox2的蛋白表达。评估Oct4和Sox2的蛋白表达水平及其与TSCC患者临床病理特征和生存情况的关系。在51例TSCC病例中，Oct4和Sox2的阳性表达主要位于肿瘤细胞核。Oct4表达强阳性27例（53%），弱阳性16例（31%），阴性8例（16%）；而Sox2表达强阳性25例（49%），弱阳性22例（43%），阴性4例（8%）。Oct4和Sox2表达水平与TSCC的组织学分级显著相关（分别为0.004、0.006），与年龄、性别、T分期、吸烟和饮酒状况无关（P>0.05），但Oct4表达水平与淋巴结转移显著相关（P=0.001）。Sox2表达水平与淋巴结转移无关（P>0.05）。Sox2的表达与Oct4显著相关（P=0.001）。Oct4和Sox2表达与TSCC患者的总生存期较差相关（分别为P=0.001、P=0.002），Oct4和Sox2表达较高的病例总生存期最差（P=0.001）。Sox2表达和淋巴结转移是TSCC患者总生存期的独立预后因素（分别为P=0.02、P=0.001）。Sox2对总生存期有独立的预后影响，提示Sox2表达可能是预测TSCC患者预后的有用指标。
