Key Laboratory of Major Diseases in Children and National Key Discipline of Pediatrics (Capital Medical University), Ministry of Education, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Nan Lishi Road, Beijing, 100045, China.
Eur J Clin Microbiol Infect Dis. 2012 Mar;31(3):217-23. doi: 10.1007/s10096-011-1296-9. Epub 2011 Jun 4.
The objective of this paper was to investigate the sequence types (STs) and diversity of surface antigen pneumococcal surface protein A (PspA) in 171 invasive Streptococcus pneumoniae isolates from Chinese children. A total of 171 pneumococci isolates were isolated from Chinese children with invasive pneumococcal diseases (IPD) in 11 hospitals between 2006 and 2008. The pneumococci samples were characterized by serotyping, PspA classification, and multilocus sequence typing (MLST). The PspA of these strains could be assigned to two families. The PspA family 2 was the most common (120/171, 70.1%). No PspA family 3 isolates were detected. Family 1 could be subdivided into two clades, with 42 strains in clade 1 and 9 strains in clade 2, and family 2 could be subdivided into clades 3, 4, and 5, which respectively contained 5, 21, and 14 strains. In total, 65 STs were identified, of which ST320 (30/171, 17.5%), ST271 (23/171, 13.5%), and ST876 (18/171, 10.5%) were the most common types. PspA family 2 and family 1 were dominant among pneumococcal clones isolated from Chinese children with invasive disease. The strains with the same ST always presented in the same PspA family.
本研究旨在调查 171 株来自中国儿童侵袭性肺炎链球菌（Spn）分离株的表面抗原肺炎球菌表面蛋白 A（PspA）的序列型（ST）和多样性。2006 年至 2008 年间，11 家医院从中国儿童侵袭性肺炎球菌病（IPD）患者中分离出 171 株肺炎球菌。采用血清分型、PspA 分类和多位点序列分型（MLST）对肺炎球菌进行了特征分析。这些菌株的 PspA 可分为 2 个家族。PspA 家族 2 最为常见（120/171，70.1%）。未检测到 PspA 家族 3 分离株。家族 1 可进一步细分为 2 个分支，分支 1 有 42 株，分支 2 有 9 株，家族 2 可进一步细分为分支 3、4 和 5，分别包含 5、21 和 14 株。共鉴定出 65 种 ST，其中 ST320（30/171，17.5%）、ST271（23/171，13.5%）和 ST876（18/171，10.5%）最为常见。PspA 家族 2 和家族 1 是从中国儿童侵袭性疾病分离株中占优势的克隆。具有相同 ST 的菌株始终存在于相同的 PspA 家族中。
